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Studies on thermoluminescence, delayed light emission and oxygen evolution from photosynthetic materials: UV effects

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Abstract

The effect of ultraviolet light on thermoluminescence, oxygen evolution and the slow component of delayed light has been investigated in chloroplasts and Pothos leaves. All peaks including peak V (48°C) were inhibited by UV. However, the peak at 48°C which was induced by DCMU was enhanced following UV irradiation of chloroplasts at ambient temperature (23°C) whereas peak II (-12°C) and peak III (10°C) which were also induced by DCMU were inhibited. Chloroplasts treated with DCMU and dark incubated for several minutes at ambient temperature prior to recording of glow curves have also shown enhancement of peak at 48°C. A slow component of delayed light and photosystem II activity of chloroplasts were inhibited by UV whereas photosystem I activity was marginally affected. These results corroborate involvement of photosystem II in generating thermoluminescence and slow components of delayed light in photosynthetic materials.

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Abbreviations

DCIP:

Dichlorophenol Indophenol

DCMU:

3-(3,4-dichlorophenyl)-1,1-dimethylurea

DCQ:

2,6 Dichloro-p-benzoquinone

DLE:

delayed light emission

MOPS:

Morpholino propane sulfonic acid

PSI:

Photosystem I

PS II:

Photosystem II

TL:

thermoluminescence

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  1. Molecular Biology and Agriculture Division, Bhabha Atomic Research Centre, Trombay, 400 085, Bombay, India

    T. S. Desai

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Desai, T.S. Studies on thermoluminescence, delayed light emission and oxygen evolution from photosynthetic materials: UV effects. Photosynth Res 25, 17–24 (1990). https://doi.org/10.1007/BF00051732

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  • DOI: https://doi.org/10.1007/BF00051732

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