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Micropropagation of mature siberian elm in two steps

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Abstract

A simple and reliable two-step micropropagation system was developed for mature Siberian elm (Ulmus pumila L.). Shoot-tip cultures were initiated and multiplied on a modified Murashige and Skoog medium supplemented with 1 μM benzyladenine, B5 vitamins, solidified with 0.22% Gelrite, and subcultured weekly. Proliferated shoots 2–3 cm in length rooted successfully in sterile and non-sterile soil mix at 100% efficiency. Healthy plants were acclimatized to the ambient environment.

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Abbreviations

2,4-D:

2,4-dichlorophenoxyacetic acid

BA:

benzyladenine

IBA:

indole-3-butyric acid

MS:

Murashige & Skoog medium (1962)

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Authors and Affiliations

  1. Department of Plant Sciences, North Dakota State University, 58105, Fargo, ND, USA

    Zong-Ming Cheng

  2. Department of Genetics, University of Wisconsin-Madison, 53706, Madison, WI, USA

    Nian-Qing Shi

Authors
  1. Zong-Ming Cheng
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  2. Nian-Qing Shi
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Cheng, ZM., Shi, NQ. Micropropagation of mature siberian elm in two steps. Plant Cell Tiss Organ Cult 41, 197–199 (1995). https://doi.org/10.1007/BF00051592

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  • DOI: https://doi.org/10.1007/BF00051592

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