Abstract
Plantlet development from in vitro cultures of Hopea odorato Roxb. is described. Embryos excised from seeds and cultured on Gamborg's B5 or modified Murashige and Skoog (MS) medium with benzyladenine (BA, 2.2–22.2 μM) produced axillary shoots at cotyledonary and/or stem nodes. Shoot production was greatest in germinated embryos on modified MS medium with 8.9 μM BA. Excised axillary shoots formed few buds when cultured on medium with BA and limited root development occurred on Woody Plant Medium with naphthaleneacetic acid. Nodal explants from aseptically grown plantlets sprouted axillary shoots in modified MS medium with BA.
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Abbreviations
- BA:
-
6-benzyladenine
- 2,4-d :
-
2,4-dichlorophenoxyacetic acid
- GA3 :
-
gibberellic acid
- IBA:
-
indolebutyric acid
- NAA:
-
naphthaleneacetic acid
- PVPP:
-
polyvinylpolypyrrolidone
References
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Scott, E.S., Rao, A.N. & Loh, C.S. Preliminary studies of micropropagation of Hopea odorata, a dipterocarp tree. Plant Cell Tiss Organ Cult 41, 193–196 (1995). https://doi.org/10.1007/BF00051591
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DOI: https://doi.org/10.1007/BF00051591