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Regeneration of Picea omorika plants via organogenesis

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Abstract

Picea omorika plants were regenerated from embryo and seedling shoot tip cultures. Adventitious and axillary shoots were produced on 1/2 MS medium containing benzyladenine and kinetin. Benzyladenine was more effective in bud induction, whereas kinetin hastened shoot development. Excised shoots were elongated on 1/3 MS medium without growth regulators, multiplied with kinetin and rooted with or without indole-3-butyric acid.

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Abbreviations

BA:

N6-benzyladenine

2IP:

N 6-(Δ2-isopenteny) adenine

NAA:

α-naphthaleneacetic acid

IAA:

indole-3-acetic acid

IBA:

indole-3-butyric acid

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Author notes

  1. Zinka Buturović-Derić

    Present address: Tasman Forestry Ltd., Centre for Advanced Forest Biotechnology, Te Teco, PO Box 149, Bay of Plenty, New Zealand

Authors and Affiliations

  1. Department of Molecular Biology, Faculty of Science, University of Zagreb, Rooseveltov trg 6, 41000, Zagreb, Croatia

    Branka Kolevska-Pletikapić & Zinka Buturović-Derić

Authors
  1. Branka Kolevska-Pletikapić
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  2. Zinka Buturović-Derić
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Kolevska-Pletikapić, B., Buturović-Derić, Z. Regeneration of Picea omorika plants via organogenesis. Plant Cell Tiss Organ Cult 41, 189–192 (1995). https://doi.org/10.1007/BF00051590

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  • DOI: https://doi.org/10.1007/BF00051590

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