Skip to main content
SpringerLink
Log in

Tissue culture response in various wild and cultivated Solanum germplasm accessions for exploitation in potato breeding

  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

The response to different in vitro methods for use in potato breeding has been evaluated in 11 genotypes of 5 Solanum species, S. etuberosum, S. lycopersicoides, S. maglia, S. rickii, and S. tuberosum. Callus induction and growth, and shoot regeneration were strongly influenced by the genotype, explant source, and medium utilized. Furthermore, considerable differences among the 11 genotypes were found both in plating efficiency and shoot regeneration from protoplast culture. Some interesting correlations were found between different tissue culture responses, suggesting linkage and/or pleiotropic effect of genes. The potential application to potato breeding of the in vitro techniques analyzed is discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

BA:

6-benzylaminopurine

GA3 :

gibberellic acid

NAA:

naphthaleneacetic acid

MS:

Murashige & Skoog (1962)

2,4-d :

dichlorophenoxyacetic acid

References

  • Abe T, Nakashima H, Mitsui K, Mikami T & Shimamoto Y (1991) Tissue culture response of Beta germplasm: callus induction and plant regeneration. Plant Cell Tiss. Org. Cult. 27: 123–127

    Google Scholar 

  • Barsby TL & Shepard JF (1983) Regeneration of plants from mesophyll protoplasts of Solanum species of the Etuberosa group. Plant Sci. Lett. 31: 101–105

    Google Scholar 

  • Barsby TL, Shepard JF, Kemble RJ & Wong R (1984) Somatic hybridization in the genus Solanum: S. tuberosum and S. brevidens. Plant Cell Rep. 3: 165–167

    Google Scholar 

  • Cardi T, Puite KJ & Ramulu KS (1990) Plant regeneration from mesophyll protoplasts of Solanum commersonii Dun. Plant Sci. 70: 215–221

    Google Scholar 

  • Cardi T, Iannamico V, D'Ambrosio F, Filippone E & Lurquin PF (1992a) Agrobacterium-mediated genetic transformation of Solanum commersonii Dun. Plant Sci. 87: 179–189

    Google Scholar 

  • Cardi T, Carputo D & frusciante L (1992b) In vitro shoot regeneration and chromosome doubling in 2X and 3X potato clones. Am. Potato J. 69: 1–12

    Google Scholar 

  • Cardi T, D'Ambrosio F, Consoli D, Puite KJ & Ramulu KS (1993a) Production of somatic hybrids between frost tolerant Solanum commersonii and S. tuberosum: characterization of hybrid plants. Theor. Appl. Genet. 87: 193–200

    Google Scholar 

  • Cardi T, Iannamico V, D'Ambrosio F, Filippone E & Lurquin PF (1993b) In vitro regeneration and cytological characterization of shoots from leaf explants of three accessions of Solanum commersonii. Plant Cell Tiss. Org. Cult. 34: 107–114

    Google Scholar 

  • Coleman M, Waugh R & Powell R (1990) Genetical analysis of in vitro cell and tissue culture response in potato. Plant Cell Tiss. Org. Cult. 23: 181–186

    Google Scholar 

  • Derks FHM (1992) Organelle transfer by protoplast fusion in Solanaceae. Proefschrift Vrije Universiteit te Amsterdam, Ponsen & Looijen, Wageningen

    Google Scholar 

  • Fish N & Jones MGK (1988) A comparison of tissue culture response between related tetraploid and dihaploid S. tuberosum genotypes. Plant Cell Tiss. Org. Cult. 15: 201–210

    Google Scholar 

  • Foulger D & Jones MGK (1986) Improved efficiency of genotype-dependent regeneration from protoplasts of important potato cultivars. Plant Cell Rep. 5: 72–76

    Google Scholar 

  • Haberlach GT, Cohen BA, Reichert NA, Towill LE & Helgeson JP (1985) Isolation, culture and regeneration of protoplasts from potato and several related Solanum species. Plant Sci. 39: 67–74

    Google Scholar 

  • Kaburu M'Ribu H & Veilleux RE (1990) Effect of genotype, explant, subculture interval, and environmental conditions on regeneration of shoots from in vitro monoploids of a diploid potato species, Solanum phureja Juz & Buk. Factors affecting shoot regeneration of monoploid potato. Plant Cell Tiss. Org. Cult. 23: 171–179

    Google Scholar 

  • Karp A, Risiott R, Jones MJK & Bright SWJ (1984). Chromosome doubling in monohaploid and dihaploid potatoes by regeneration from cultured leaf explants. Plant Cell Tiss. Org. Cult. 3: 363–373

    Google Scholar 

  • Koornneef M, Hanart CJ & Martinelli L (1987) A genetical analysis of cell culture traits in tomato. Theor. Appl. Genet. 74: 633–641

    Google Scholar 

  • Locy RD (1983) Callus formation and organogenesis by explants of six Lycopersicon species. Can. J. Bot. 61: 1072–1079

    Google Scholar 

  • Masson T, Lancelin D, Bellini C, Leraf M, Guerce P & Pelletier G (1989) Selection of somatic hybrids between diploid clones of potato (Solanum tuberosum L.) transformed by direct gene transfer. Theor. Appl. Genet. 78: 153–159

    Google Scholar 

  • Morpurgo R (1991) Correlation between potato clones grown in vitro and in vivo under sodium chloride stress conditions. Plant Breeding 107: 80–82

    Google Scholar 

  • Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497

    Google Scholar 

  • Narassiman R & Rangasamy SRS (1993) Correlation and heritability for callus induction and regeneration in in vitro anther culture of indica-japonica rice hybrids. J. Genet. & Breed. 47: 187–190

    Google Scholar 

  • Nadolska-Orczyk A & Malepszy S (1989) In vitro culture of Cucumis sativus L. Genes controlling plant regeneration. Theor. Appl. Genet. 78: 836–840

    Google Scholar 

  • Ochatt SJ & Caso OH (1986) Differential requirements among tissue source in Solanum tuberosum L. ssp andigena callus cultures. Turrialba 36: 363–368

    Google Scholar 

  • O'Connell MA & Hanson MR (1986) Regeneration of somatic hybrid plants formed between Lycopersicon esculentum and Solanum rickii. Theor. Appl. Genet. 72: 59–65.

    Google Scholar 

  • Ohky S, Bigot C & Mousseau J. (1978) Analysis of shoot-forming capacity in vitro in two lines of tomato (Lycopersicon esculentum Mill.) and their hybrids. Plant Cell Physiol. 19: 27–42

    Google Scholar 

  • Peloquin SJ, Yerk GL, Werner JE & Darmo E (1989) Potato breeding with haploids and 2n gametes. Genome 31: 1000–1004

    Google Scholar 

  • Puite KJ, Roest S & Pijnacker LP (1986) Somatic hybrids potato plants after electrofusion of diploid Solanum tuberosum and Solanum phureja. Plant Cell Rep. 5: 262–265

    Google Scholar 

  • Ramulu KS, Dijkhuis P, Hanisch Ten Cate Ch & De Groot B (1985) Patterns of DNA and chromosome variation during in vitro growth in various genotypes of potato. Plant Sci. 41: 69–78

    Google Scholar 

  • Serraf I, Sihachakr D, Ducreux G, Brown SG, Allot M, Barghi N & Rossignol L (1991) Interspecific somatic hybrids in potato by protoplast electrofusion. Plant Sci. 76: 115–126

    Google Scholar 

  • Steele RGD & Torrie JH (1980) Principles and procedures of statistics. A biometrical approach. Second ed. McGraw-Hill Book Co., New York

    Google Scholar 

  • Taylor TE & Veilleua RE (1992) Inheritance of competencies for leaf disc regeneration, anther culture, and protoplast culture in Solanum phureja and correlations among them. Plant Cell Tiss. Org. Cult. 31: 95–103

    Google Scholar 

  • Tan MMC, Colijn-Hooymans CM, Lindhout WH & Kool AJ (1987) A comparison of shoot regeneration from protoplasts and leaf discs of diploid genotypes of the cultivated tomato. Theor. Appl. Genet. 75: 105–108

    Google Scholar 

  • Tavazza R, Ordas RJ & Ancora G (1988) Procedure for regeneration of plants from cell suspension protoplasts of tetraploid potato (Solanum tuberosum L.) cv. Desiree. Plant Sci. 58: 223–230

    Google Scholar 

  • Veronneau H, Lavoie G & Cappadocia M (1992) Genetic analysis of anther culture and leaf disc culture in two clones of Solanum chacoense Bitt. and their reciprocal hybrids. Plant Cell Tiss. Org. Cult. 30: 199–209

    Google Scholar 

  • Vries SE, Jacobsen E, Jones MGK, Looner AEHM, Tempelaar MJ, Wijbrabdi JW & Feenstra WJ (1987) Somatic hybridization of amino-acid analogue-resistant cell lines of potato (Solanum tuberosum L.) by electrofusion. Theor. Appl. Genet. 73: 451–458

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Agronomy and Plant Genetics, University of Naples, Via Universita' 100, 80055, Portici, Italy

    Domenico Carputo, Giovanni Ferraiolo & Luigi Frusciante

  2. Research Centre for Vegetable Breeding, National Research Council (CNR), Via Universita' 133, 80055, Portici, Italy

    Teodoro Cardi

  3. Istituto Agronomico per l'Oltremare (IAO), Via Cocchi 4, 50100, Firenze, Italy

    Tiberio Chiari

Authors
  1. Domenico Carputo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Teodoro Cardi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Tiberio Chiari
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Giovanni Ferraiolo
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Luigi Frusciante
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Carputo, D., Cardi, T., Chiari, T. et al. Tissue culture response in various wild and cultivated Solanum germplasm accessions for exploitation in potato breeding. Plant Cell Tiss Organ Cult 41, 151–158 (1995). https://doi.org/10.1007/BF00051584

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00051584

Key words

Search

Navigation