Abstract
Micropropagation of 21 species of Restionaceae and the closely related
monotypic families Anarthriaceae and Ecdeiocoleaceae is discussed. Multiplication rates ranged from 2-fold to 14-fold each 4–6 week subculture passage, with most species averaging 3–5-fold. The majority of taxa preferred half-strength Murashige and Skoog basal media with 1 μM benzyladenine, with certain species requiring other specific treatments (e.g. Woody Plant medium). Approximately half of the species produced roots successfully (i.e. >50%) in vitro on half-strength MS with no growth regulators (or no auxins), or initiated roots after transfer to soil; other species required a longer (6–7 week) incubation on quarter-strength MS medium for initiation to occur. This paper describes the first successful micropropagation of these taxa with application for horticultural development of this important southern-hemisphere family.
Similar content being viewed by others
Abbreviations
- MS:
-
Murashige & Skoog (1962) basal medium
- 1/4 MS:
-
quarter-strength MS (minerals only)
- 1/2 MS:
-
half-strength MS (minerals only)
- BA:
-
benzyladenine
- WPM:
-
woody plant medium (Lloyd and McCown 1980)
- GA3 :
-
gibberellic acid
- TDZ:
-
thidiazuron
- IBA:
-
indolebutyric acid
References
Bunn E & Dixon KW (1992) Micropropagation of the Pineapple Lily, Dasypogon hookeri J. Drumm. HortScience 27: 369
Johnson LAS & Briggs BG (1983) Restionaceae In: Morley B D & Toelken H R (Eds) ‘Flowering Plants in Australia’ (p 371–373). Rigby: Adelaide, Australia
Lloyd GB & McCown BH (1980) Tissue culture of rhododendrons and formulations of woody plant medium. Comb. Proc. Int. Plant Prop. Soc. 30: 421–427
Meney KA (1993) Functional aspects of the growth, development and reproduction of southern hemisphere rushes (Restionaceae). Phd thesis. University of Western Australia
Meney KA & Dixon KW (1995) In vitro propagation of Western Australian Rushes (Restionaceae) and related families by embryo culture. I. in vitro embryo growth. Plant Cell Tiss. Org. Cult. (this volume)
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 15: 473–497
Pate JS, Meney KA & Dixon KW (1991) Contrasting growth and morphological characteristics of fire-sensitive (obligate seeder) and fire-resistant (resprouter) species of Restionaceae (S. Hemisphere restiads) from S.W. Western Australia. Aust. J. Bot. 39: 505–525
Rossetto M, Dixon KW & Bunn E (1992a) Aeration: A simple method to control vitrification and improve in vitro culture of rare Australian plants. In vitro Cell. Dev. Biol. 28P: 192–196
Rossetto M, Dixon KW, Meney KA & Bunn E (1992b) In vitro propagation of Chinese puzzle (Caustis dioica Cyperaceae) — a commercial sedge species from Western Australia. Plant Cell Tiss. Org. Cult. 30: 65–67
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Meney, K.A., Dixon, K.W. In vitro propagation of Western Australian Rushes (Restionaceae and related families) by embryo culture. Part 2. Micropropagation. Plant Cell Tiss Organ Cult 41, 115–124 (1995). https://doi.org/10.1007/BF00051580
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00051580