Abstract
Mutation of the core pentamer, CCGAC, of two putative low temperature responsive elements (LTREs) in the 5′-proximal region of the winter Brassica napus cold-induced gene BN115 was carried out. Analyses of transient expression of the resultant mutated BN115 promoter-GUS fusions revealed the loss of low-temperature regulation by the promoter. This indicates that the CCGAC sequence is critical to the low-temperature response in the BN115 gene. In contrast, mutation of two G-boxes, CACGTG, staggered between the LTREs in the same region of the promoter did not alter cold-inducible gene expression. Replacement of a possible enhancer region of the BN115 promoter with the enhancer from the CaMV 35S promoter resulted in a several-fold increase in low temperature-induced GUS activity.
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Jiang, C., Iu, B. & Singh, J. Requirement of a CCGAC cis-acting element for cold induction of the BN115 gene from winter Brassica napus . Plant Mol Biol 30, 679–684 (1996). https://doi.org/10.1007/BF00049344
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DOI: https://doi.org/10.1007/BF00049344