Abstract
The inactivated O2-evolving center of Tris-washed chloroplasts was reactivated by DCPIP-treatment and photoreactivation in the presence of Mn2+, Ca2+, DTT and weak light. Many electron donors (Asc and reduced DCPIP, etc.) were found to be suitable substitutes for DTT. By studying the anaerobic inhibition of the reactivation, the electron acceptors O2, NADP+, etc. were also found to be essential factors in photoreactivation. Weak light stimulated the chloroplast electron transport from the above-mentioned electron donors to the electron acceptor and effected the photoreactivation. More than 280 electrons were transported to NADP+ in the anaerobic photoreactivation of one unit of an O2-evolving center with 400 Chl. Electron transport in the reactivation was inhibited by omitting DTT or Mn2+ ion, and by adding DCMU. The photoreactivated chloroplasts incorporated about 2 Mn by 400 Chl. Omission of DTT in the reactivation caused chloroplasts in the weak light to bind large amounts of excess Mn.
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Abbreviations
- Asc:
-
ascorbate
- Chl:
-
chlorophyll
- DCPIP:
-
2, 6-dichlorophenol indophenol
- DPC:
-
diphenyl carbazide
- DTT:
-
dithiothreitol
- Fd:
-
ferredoxin
- STN:
-
a chloroplast preparation medium, containing 0.4 M sucrose, 0.05 M Tris-Cl and 0.01 M NaCl (pH 7.8 and 8.0)
- TMPD:
-
tetramethyl-p-phenylenediamine
References
Callahan FE and Cheniae GM (1985) Studies on the photoactivation of the water-oxidizing enzyme. I. Processes limiting photoactivation in hydroxylamine-extracted leaf segments. Plant Physiol 79: 777–786
Cheniae GM and Martin IF (1967) Photoreactivation of manganese catalyst in photosynthetic oxygen evolution. Biochem Biophys Res Commun 28: 89–95
Cheniae GM and Martin IF (1969) Photoreactivation of manganese catalyst in photosynthetic oxygen evolution. Plant Physiol 44: 351–360
Cheniae GM and Martin IF (1970) Sites of function of manganese within photosystem II. Roles in O2 evolution and system II. Biochim Biophys Acta 197: 219–239
Cheniae GM and Martin IF (1971) Photoactivation of the manganese catalyst of O2 evolution. I. Biochemical and Kinetic asepcts. Biochim Biophys Acta 253: 167–181
Cheniae GM and Martin IF (1972) Effect of hydroxylamine on photosystem II. II. Photoreversal of the NH2OH destruction of O2 evolution. Plant Physiol 50: 87–94
Cheniae GM and Martin IF (1973) Absence of oxygen-evolving capacity in dark-grown Chlorella: The photoactivation of oxygen-evolving centers. Photochem Photobiol 17: 441–159
Inoué H and Wada T (1987) Requirement of manganese for electron donation of hydrogen peroxide in photosystem II reaction center complex. Plant Cell Physiol 28: 767–773
Inoue Y, Kobayashi Y, Sakamoto E and Shibata K (1975) Multiple flash activation of the water-photolysis system in intermittently illuminated wheat leaves. Plant Cell Physiol 16: 327–336
Oku T and Tomita G (1980) Photoactivation of the latent O2-evolving center in chloroplasts isolated from dark-grown epruce seedlings. Physiol Plant 48: 99–103
Ono TA and Inoue Y (1987) Reductant-sensitive intermediates involved in multi-quantum process of photoactivation of latent O2-evolving system. Plant Cell Physiol 28: 1293–1299
Tamura N and Cheniae GM (1986) Requirement for the photoligation of Mn2+ in PS II membranes and the expression of water-oxidizing activity of the polynuclear Mn-catalyst. FEBS Lett 200: 231–236
Tamura N and Cheniae GM (1987) Photoactivation of the water-oxidizing complex in Photosystem II membranes depleted of Mn and extrinsic proteins. I. Biochemical and kinetic characterization. Biochim Biophys Acta 890: 179–194
Theg SM and Sayre RT (1979) Characterization of chloroplast manganese by electron paramagnetic resonance spectroscopy. Plant Sci Lett 16: 319–326
Vernon LP, SanPietro A and Limbach DA (1965) Photoreduction of NAD and NADP by chlorophyllin a in the presence of ascorbate: Requirement for NADP reductase. Arch Biochem Biophys 109: 92–97
Yamashita T and Tomita G (1975) Comparative study of the reactivation of oxygen evolution in chloroplasts inhibited by various treatment. Plant Cell Physiol 16: 283–296
Yamashita T, Inoue Y, Kobayashi Y and Shibata K (1978) Flash reactivation of Tris-inactivated chloroplasts. Plant Cell Physiol 19: 895–900
Yocum CF, Yerkes CT, Blankenship RE, Sharp RR and Babcock GT (1981) Stoichiometry, inhibitor sensitivity, and organization of manganese associated with photosynthetic oxygen evolution. Proc Natl Acad Sci USA 78: 7507–7511
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Yamashita, T., Kobayashi, T., Morita, Y. et al. The roles of weak light, oxygen and dithiothreitol in the photoreactivation of the oxygen evolving center of tris-washed and 2, 6-dichlorophenol indophenol-treated chloroplasts. Photosynth Res 26, 1–8 (1990). https://doi.org/10.1007/BF00048971
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DOI: https://doi.org/10.1007/BF00048971