Abstract
Immature zygotic embryos of ginseng produced somatic embryos on MS medium without growth regulators. However, in the culture of mature zygotic embryos, excision of the embryo was required for somatic embryo induction. Somatic embryos formed only on excised cotyledons without an embryo axis or on excised embryos without the plumule and radicle of the axis. This observation suggests that the axis tip of the embryo might suppress somatic embryo production although the cotyledon tissues have predetermined embryogenic competency. To clarify the role of the embryo axis on somatic embryo formation, excised plumules or radicles were placed in direct contact with the basal cut-ends of cotyledons. The adhesion of plumules or radicles highly suppressed somatic embryo formation from cotyledon explants. When an agar block containing exudate from excised plumules or radicles was placed in contact with the cut end of the cotyledon, a similar inhibition was observed. These results suggest that embryogenic competence is suppressed by endogenous inhibitors present in the axis tip of the zygotic embryo.
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Arya S, Arya ID & Eriksson T (1993) Rapid multuplication of adventitious somatic embryos of Panax ginseng Plant Cell, Tiss. Org. Cult. 34: 157–162
Borkird C, Choi JH & Sung ZR (1986) Effect of 2,4-dichlorophenoxyacetic acid on the expression of embryogenic program in carrot. Plant Physiol. 81: 1143–1146
Choi YE & Soh WY (1994a) Origin of somatic embryos from excised cotyledons of ginseng. Korean J. Plant Tiss. Cult. 21: 177–182
Choi YE & Soh WY (1994b) Origin of somatic embryo induced from cotyledons of zygotic embryos at various developmental stages of ginseng. J. Plant Biol. 37: 365–370
Geneve RL & Kester ST (1990) The initiation of somatic embryos and adventitious roots from developing zygotic embryo explants of Cercis canadensis L. cultured in vitro. Plant Cell Tiss. Org. Cult. 22: 71–76
Hu CY & Sussex IM (1971) In vitro development of embryoids on cotyledons of Ilex aquifolium. Phytomorph. 21: 103–107
Jones TJ & Rost TL (1989) The developmental anatomy and ultrastructure of somatic embryos from the rice (Oryza sativa L.) scutellum epidermal cells. Bot. Gaz. 150: 41–49
Konar RN & Nataraja K (1965) Experimental studies in Ranunculus sceleratus L. Development of embryos from the stem epidermis. Phytomorph. 15: 132–137
Kwon WS, Jung CM, Ahn SD & Choi KT (1986) Effects of growth regulators on the germination of Panax ginseng C. A. Meyer. Korean J. Ginseng Sci. 10: 159–166
Lee HS, Lee KW, Yang SG & Liu JR (1989) Plant regeneration through somatic embryogenesis from mature zygotic embryos of ginseng (Panax ginseng C. A. Meyer) and flowering of plantlets. Korean J. Bot. 32: 145–150
Lu CY & Vasil IK (1982) Somatic embryogenesis and plant regeneration in tissue cultures of Panicum maximum Jacq. Am. J. Bot. 69: 77–81
Maheswaran G & Wiliams EG (1985) Origin and development of somatic embryoids formed directly on immature embryos of Tripolium repens in vitro. Ann. Bot. 56: 619–630
Moncousin C, Favre JM & Gaspar T (1989) Early changes in auxin and ethylene productian in vine cuttings before adventitious rooting. Plant Cell Tiss. Org. Cult. 19: 235–242
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Pence VC, Hasegawa PM & Janick J (1980) Initiation and development of asexual embxyos of Theobroma cacao L. in vitro. Z. Pflanzenphysiol. 98: 1–14
Schiavone FM & Racusen RH (1990) Microsurgery reveals regional capabilities for pattern reestablishment in somatic carrot embryos Dev. Biol. 141: 211–219
Sharp WR, Sondahl MR, Caldas LS & Maraffa SB (1980) The physiology of in vitro asexual embryogenesis. Hort. Rev. 2: 268–310
Smith DL & Krikorian AD (1989) Release of somatic embryogenic potential from excised zygotic embryos of carrot and maintenance of proembryonic cultures in hormone-free medium. Amer. J. Bot. 76: 1832–1843
Wang D, Wergin WP & Zimmerman RH (1984) Somatic embryogenesis and plant regeneration from immature embryos of strawberry. HortScience Letters 4: 231–236
Williams EG & Maheswaran G (1986) Somatic embryogenesis: Factors influencing coordinated behavior of cells as an embryogenic group. Ann. Bot. 57: 443–462
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Choi, Y.E., Soh, W.Y. Effect of plumule and radicle on somatic embryogenesis in the cultures of ginseng zygotic embryos. Plant Cell Tiss Organ Cult 45, 137–143 (1996). https://doi.org/10.1007/BF00048757
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DOI: https://doi.org/10.1007/BF00048757