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An improved method for somatic plantlet production in hybrid larch (Larix × leptoeuropaea): Part 1. Somatic embryo maturation

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Abstract

Somatic embryogenesis was induced from full-sib immature zygotic embryos of hybrid larch (Larix x leptoeuropaea) that were collected at three different dates. Analysis of variance showed interaction between the collection date and the induction medium. The highest response (55%) was observed from embryos that were at the precotyledonary stage. Twelve media containing various concentrations of abscisic acid and sucrose were used to promote the development of ‘high quality’ mature somatic embryos that would undergo a period of developmental arrest. Only media supplemented with abscisic acid (20, 40, and 60 μM), indolebutyric acid (1 μM), and 0.1 or 0.2 M sucrose supported such a development. The number of mature somatic embryos produced per gram fresh weight of embryonal mass was significantly affected by the three factors tested: embryogenic line, sucrose concentration, and abscisic acid concentration. Moreover, strong interaction effects among these factors existed, complicating the formulation of a universal maturation medium that would be optimal for all embryogenic lines.

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Abbreviations

ABA:

abscisic acid

BA:

benzyladenine

IBA:

indolebutyric acid

2,4-d :

2,4-dichlorophenoxyacetic acid

EM:

embryonal mass

EPot:

embryogenic potential

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Authors and Affiliations

  1. INRA Station d'Amélioration des Arbres Forestiers, Centre de recherches d'Orléans, Ardon, F-45 160, Oliver, France

    M. A. Lelu & C. Bastien

  2. Petawawa National Forestry Institute, Forestry Canada, P.O. Box 2000, KOJ 1JO, Chalk River, Ontario, Canada

    K. Klimaszewska, C. Ward & P. J. Charest

Authors
  1. M. A. Lelu
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  2. C. Bastien
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  3. K. Klimaszewska
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  4. C. Ward
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  5. P. J. Charest
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Lelu, M.A., Bastien, C., Klimaszewska, K. et al. An improved method for somatic plantlet production in hybrid larch (Larix × leptoeuropaea): Part 1. Somatic embryo maturation. Plant Cell Tiss Organ Cult 36, 107–115 (1994). https://doi.org/10.1007/BF00048321

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  • DOI: https://doi.org/10.1007/BF00048321

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