Abstract
Photosynthesis and phosphoenolpyruvate carboxylase activity were investigated in 5, 10 and 28 day-old micropropagated strawberry plantlets (Fragaria x ananassa Duch. cv Kent) rooted in vitro with different levels of sucrose (0, 1, 3 and 5%) on cellulose plugs (Sorbarods). The photosynthetic capability was influenced by the level of sucrose in the culture medium with the largest rates of photosynthesis corresponding to the cultures with 0 and 1% sucrose. The apparent quantum yield and the ratio of variable fluorescence to maximum fluorescence were also reduced in plantlets cultured with 3 or 5% sucrose as compared to those with 0 or 1%. Phosphoenolpyruvate carboxylase activity was largest 5 and 10 days after the onset of culture and decreased in the absence of sucrose in the culture medium. At 28 days after the onset of culture, the activity of this carboxylating enzyme was lower than at the beginning of culture and independent of the concentration of sucrose in the culture medium. Phosphoenolpyruvate carboxylase appears to be an important carboxylating enzyme in micropropagated plantlets.
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References
Aoki S & Oda M (1988) Sensing of photosynthetic capacities of seedlings of lettuce with chlorophyll fluorescence. Acta Hort. 230: 363–370
Aoyagi K & Bassham JA (1986) Appearance and accumulation of C4 carbon pathway enzymes in developing wheat leaves. Plant Physiol. 80: 334–340
Azcòn-Bieto J (1983) Inhibition of photosynthesis by carbohydrates in wheat leaves. Plant Physiol. 73: 681–686
Azcòn-Bieto J (1986) The control of photosynthetic gas exchange by assimilate accumultion in wheat. In: Marcelle R, Clijsters H & Van Pouke M (Eds) Biological Control of Photosynthesis (pp 231–240). Martinus Nijhoff Publishers, Dordrecht
Bender L, Kumar LA & Neumann KH (1985) On the photosynthetic system and assimilate metabolism of Daucus and Arachis cell cultures. In: Neumann KH, Barz W & Reinhart E (Eds) Primary and Secondary Metabolism of Plant Cell Cultures (pp 22–42). Springer-Verlag, Berlin
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein using the principle of protein-dye binding. Anal. Biochem. 72: 248–254
Brainerd KE, Fuchigami LH, Kwiatkowski S & Clark CS (1981) Leaf anatomy and water stress of aseptically cultured ‘Pixy’ plum grown under different environments. HortScience 16: 173–175
Capellades M, Lemeur L & Debergh P (1990) Kinetics of Chlorophyll Fluorescence in Micropropagated Rose Shootlets. Photosynthetica 24: 190–193
Capellades M, Lemeur L & Debergh P (1991) Effects of sucrose on starch accumulation and rate of photosynthesis in Rosa culture in vitro. Plant Cell Tiss. Org. Cult. 25: 21–26
Desjardins Y, Gosselin A & Yelle S (1987) Acclimatization of ex vitro strawberry plantlets in CO2-enriched environments and supplementary lighting. J. Amer. Soc. Hort. Sci. 112: 846–851
Desjardins Y, Laforge F, Lussier C & Gosselin A (1988) Effects of CO2 enrichment and high photosynthetic photon flux on the development of autotrophy and growth of tissue-cultured strawberry, raspberry and asparagus plants. Acta Hort. 230: 45–47
Grout BWW (1988) Photosynthesis of regenerated plantlets in vitro, and the stress of transplanting. Acta Hort. 230: 129–135
Kozai T (1991) Photoautotrophic and photomixotrophic growth of strawberry plantlets in vitro and changes in nutrient composition of the medium. Plant Cell Tiss. Org. Cult. 25: 107–115
Kozai T, Kubota C & Watanabe I (1988) Effects of basal medium composition on the growth of carnation plantlets in auto- and mixotrophic tissue culture. Acta Hortic. 230: 119–126
Kumar PP, Bender L & Thorpe TA (1988) Activities of ribulose bisphosphate carboxylase and phosphoenolpyruvate carboxylase and 14C-bicarbonate fixation during in vitro culture of Pinus radiata cotyledons. Plant Physiol. 87: 675–679
Langford PJ & Wainwright H (1988) Influence of sucrose concentration on the photosynthetic ability of in vitro grown rose shoots. Acta Hort. 22: 305–308
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant. 15: 473–497
Short KC, Warburton J & Roberts AV (1987) In vitro hardening of cultured cauliflower and chrysanthemum plantlets to humidity. Acta Hort. 212: 329–334
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Hdider, C., Desjardins, Y. Effects of sucrose on photosynthesis and phosphoenolpyruvate carboxylase activity of in vitro cultured strawberry plantlets. Plant Cell Tiss Organ Cult 36, 27–33 (1994). https://doi.org/10.1007/BF00048312
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DOI: https://doi.org/10.1007/BF00048312