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Indole alkaloids in clonal propagules of Rauwolfia serpentina benthe ex kurz

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Abstract

Plantlets were succesfully regenerated from shoot cultures of Rauwolfia serpentina initiated from auxillary meristems on medium containing BA (4.44 μM) + NAA (0.54 μM). Rooting was initiated in White's basal medium supplemented with NAA (0.54 μM). Tissue culture derived piants of R. serpentina (RSTC) were similar to normal plants (RS) in their morphological characteristics and chemical consitution. The biomass of the RSTC plants was higher (47.11 gms) than the normal plant (18.23 gms) on a dry weight basis. Five RSTC plants were cloned and the cloned plants were similar in biomass and alkaloid content to the normal plant.

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Abbreviations

BA:

benzyl adenine

NAA:

naphthalene acetic acid

MS:

Murashige and Skoog

TLC:

thin layer chromatography

HPLC:

high performance liquid chromatography

F.W.:

fresh weight

D.W.:

dry weight

RS:

normal field grown plant established from stem cuttings

RSTC:

tissue culture plants established from shoot cultures

R:

reserpine

Aj:

ajmalicine

A:

ajmaline

S:

serpentine

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Authors and Affiliations

  1. Biotechnology Division, Bhabha Atomic Research Centre, Trombay, 4000 85, Bombay, India

    G. Roja & M. R. Heble

Authors
  1. G. Roja
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  2. M. R. Heble
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Roja, G., Heble, M.R. Indole alkaloids in clonal propagules of Rauwolfia serpentina benthe ex kurz. Plant Cell Tiss Organ Cult 44, 111–115 (1996). https://doi.org/10.1007/BF00048187

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  • DOI: https://doi.org/10.1007/BF00048187

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