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Protoplast culture and somaclonal variability of species of series Juglandifolia

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Abstract

Mesophyll protoplasts of species of series Juglandifolia (Solanum rickii, S. lycopersicoides, S. ochranthum and S. juglandifolium) were isolated and cultured in liquid nutrient media TM-2 or KM8P. The cell colonies formed were transferred onto agar-solidified media TM-3 or GM, and 10 to 15 days later onto TM-4, PRM, MS3ZG, KK or C regeneration media. Formation of the shoots for S. rickii and S. lycopersicoides was observed after 30 to 35 days on regeneration medium. The regenerated shoots were rooted on hormone-free MS medium. Morphological and cytogenetic analyses have shown that somaclonal variants might arise in the course of plant regeneration from protoplasts of these species.

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Abbreviations

BAP:

6-benzylaminopurine

2, 4-D:

2, 4 dichlorophenoxyacetic acid

NAA:

α-naphthaleneacetic acid

GA3 :

gibberellic acid

IAA:

indole-3-acetic acid

MS:

Murashige and Skoog medium

B5:

Gamborg medium

TRIS:

tris(hydroxymethyl)aminomethane

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Author notes

  1. Y. Y. Gleba

    Present address: American Cyanamid Company, P.O.B. 400, 08543-0400, Princeton, NJ, USA

Authors and Affiliations

  1. Institute of Cell Biology and Genetic Engineering, National Academy of Sciences of Ukraine, Zabolotnogo str. 148, 252022, DSP-22, Kiey, Ukraine

    A. S. Kochevenko, Y. I. Ratushnyak & Y. Y. Gleba

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  1. A. S. Kochevenko
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  2. Y. I. Ratushnyak
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  3. Y. Y. Gleba
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Kochevenko, A.S., Ratushnyak, Y.I. & Gleba, Y.Y. Protoplast culture and somaclonal variability of species of series Juglandifolia . Plant Cell Tiss Organ Cult 44, 103–110 (1996). https://doi.org/10.1007/BF00048186

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  • DOI: https://doi.org/10.1007/BF00048186

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