Skip to main content
SpringerLink
Log in

Development of a simple particle bombardment device for gene transfer into plant cells

  • Original Research Paper
  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

A simple particle bombardment device was designed, constructed and shown to be efficient for the delivery of DNA into plant cells. High levels of transient β-glucuronidase expression were observed in alfalfa suspension-cultured cells and embryogenic soybean suspension-cultured cells. Expression of β-glucuronidase in alfalfa suspension-cultured cells was used to optimize the bombardment conditions for the device. Transient gene expression in alfalfa was found to be dependent on the state of the target tissue, the size of particles employed, the helium pressure used to accelerate the particles and the distance travel led by the tungsten particles carrying DNA.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

2,4-d :

2,4-dichlorophenoxyacetic acid

GUS:

β-glucuronidase

MS:

Murashige & Skoog (1962) medium

References

  • Atanassov A & Brown DCW (1984) Plant regeneration from suspension culture and mesophyll protoplasts of Medicago sativa L. Plant Cell Tiss. Org. Cult. 3: 149–162

    Google Scholar 

  • Finer JJ, Vain P, Jones MW & McMullen M (1993) Development of particle inflow gun for DNA delivery to plant cells. Plant Cell Rep. 11: 323–328

    Google Scholar 

  • Gamborg OL, Miller RA & Ojima K (1968) Nutrient requirement of suspension cultures of soybean root cells. Exp. Cell Res. 50: 151–158

    Google Scholar 

  • Gray DJ & Finer JJ (1993) Development and operation of five particle guns for introduction of DNA into plant cells. Plant Cell Tiss. Org. Cult. 33: 219

    Google Scholar 

  • Jefferson R (1988) Plant reporter genes: The Gus gene fusion system. In: Setlow JK & Hollaender A (Ed) Genetic Engineering: Principles and Methods (pp 247–263). New York, Plenum Press

    Google Scholar 

  • Kikkert JR (1993) The Biolistic PDS-1000/He device. Plant Cell Tiss. Org. Cult. 33: 221–226

    Google Scholar 

  • Klein TM, Wolf ED, Wu R & Sanford JC (1987) High-velocity microprojectiles for delivering nucleic acids into living cells. Nature 327: 70–73

    Google Scholar 

  • Lazzeri PA, Hildebrand DF & Collins GB (1985) A procedure for plant regeneration from immature cotyledon tissue of soybean. Plant Mol. Biol. Rep. 3: 160–167

    Google Scholar 

  • McCabe DE & Christou P (1993) Direct DNA transfer using electric discharge particle acceleration (ACCELL technology). Plant Cell Tiss. Org. Cult. 33: 227–236

    Google Scholar 

  • McCabe DE, Swain WF, Martinell BJ & Christou P (1988) Stable transformation of soybean (Glycine max) by particle acceleration. Bio/Technology 6: 923–926

    Google Scholar 

  • Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497

    Google Scholar 

  • Oard J (1993) Development of an airgun device for particle bombardment. Plant Cell Tiss. Org. Cult. 33: 247–250

    Google Scholar 

  • Oard JH, Paige DF, Simmonds JA & Gradziel TM (1990) Transient gene expression in maize, rice, and wheat cells using an airgun apparatus. Plant. Physiol. 92: 334–339

    Google Scholar 

  • Sautter C (1993) Development of a microtargeting device for particle bombardment of plant meristems. Plant Cell Tiss. Org. Cult. 33: 251–257

    Google Scholar 

  • Sautter C, Waldner H, Neuhaus-Url G, Falli A, Neuhaus G & Potrykus I (1991) Micro-targeting: high efficiency gene transfer using a novel approach for the acceleration of micro-projectiles. Bio/Technology 9: 1080–1085

    Google Scholar 

  • Schenk B & Hildebrandt A (1972) Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Canadian Journal of Botany 50: 199–204

    Google Scholar 

  • Seki M, Komeda Y, Iida A, Yamada Y & Morikawa H (1991) Transient expression of β-glucuronidase in Arabidopsis thaliana leaves and roots and Brassica napus stems using a pneumatic particle gun. Plant Mol. Biol. 17: 259–263

    Google Scholar 

  • Vain P, Keen N, Murillo J, Rathus C, Nemes C & Finer JJ (1993) Development of the particle inflow gun. Plant Cell. Tiss. Org. Cult. 33: 237–246

    Google Scholar 

  • Weising K, Schell J & Kahl G (1988) Foreign genes in Plants: transfer, structure, expression, and application. Annu. Rev. Genet. 22: 421–477

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Plant Research Centre, Agriculture Canada, Central Experimental Farm, Ottawa, Canada

    Daniel C. W. Brown & Lining Tian

  2. Centre for Food and Animal Research, Agriculture Canada, Central Experimental Farm, Ottawa, Canada

    Del J. Buckley, Mark Lefebvre & Andy McGrath

  3. Biology Department, Carleton University, Ottawa, Canada

    Daniel C. W. Brown, Lining Tian & John Webb

  4. Crop Science Department, University of Guelph, Guelph, Canada

    Daniel C. W. Brown

Authors
  1. Daniel C. W. Brown
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Lining Tian
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Del J. Buckley
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mark Lefebvre
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Andy McGrath
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. John Webb
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Brown, D.C.W., Tian, L., Buckley, D.J. et al. Development of a simple particle bombardment device for gene transfer into plant cells. Plant Cell Tiss Organ Cult 37, 47–53 (1994). https://doi.org/10.1007/BF00048116

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00048116

Key words

Search

Navigation