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Photosynthesis in callus cultures of Choisya ternata (Rutaceae)

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Abstract

Photomixotrophic callus cultures of Mexican Orange (Choisya ternata HBK) containing about 100 μg chlorophyll g-1 fresh weight, were grown under various light regimes on a medium containing 41 mM glucose as the sole organic compound. O2 and CO2 exchanges were simultaneously recorded during a culture cycle. The data showed that the cells were photosynthetically active and switched from photomixotrophy to photoautotrophy after glucose had been used up and the culture medium was free of any organic compound. The cells were also grown under strict photoautotrophic conditions for 6 months under continuous light and 2% CO2 with monthly subcultures.

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Abbreviations

Chl:

Chlorophyll

Cin :

CO2 concentration in the culture flasks

CP:

compensation point

DR:

dark respiration

DCMU-3:

(3,4-dichlorophenyl)-1,1-dimethylurea

PEP:

phosphoenolpyruvate

Pg:

gross photosynthesis

Pn:

net photosynthesis

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Authors and Affiliations

  1. Laboratoire de Biochimie Végétale et Biologie Cellulaire. EA 1370-DRED, Faculté de Pharmacie, 37000, Tours, France

    J. Creche & M. Rideau

  2. Laboratoire de Physiologie Végétale, Faculté des Sciences, 23 rue du Dr. P. Michelon, 42023, St Etienne, France

    J. C. Leclerc

Authors
  1. J. Creche
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  2. M. Rideau
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  3. J. C. Leclerc
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Creche, J., Rideau, M. & Leclerc, J.C. Photosynthesis in callus cultures of Choisya ternata (Rutaceae). Plant Cell Tiss Organ Cult 37, 9–14 (1994). https://doi.org/10.1007/BF00048111

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  • DOI: https://doi.org/10.1007/BF00048111

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