Abstract
By sequencing cDNA clones, we have concluded that three distinct sucrose genes are expressed in rice (Oryza sativa cv. Tainong 67). When the amino acid sequences deduced from these cDNAs as well as those of known sucrose synthase are compared, the highest divergence is found in the C-termini. The most suitable DNA sequences for use as specific for the mRNA derived from these genes have been suggested.
References
Chan, HY, Ling, TY, Juang, RH, Ting, IN, Sung, HY, Su, JC: Sucrose synthase in rice plants: Growth-associated changes in tissue specific distributions. Plant Physiol 94: 1456–1461 (1990).
Chen, HJ, Sung, HY, Su, JC: Role of sucrose synthetase in sucrose-starch conversion in rice grains. Proc Natl Sci Counc ROC B 5: 165–170 (1981).
Chourey, PS: Electrophoretic analysis of sucrose synthetase proteins in the complementing heterozygotes at the Shrunken locus in maize. Theor Appl Genet 59: 231–234 (1981).
Chourey, PS, Latham, MD, Still, PE: Expression of two sucrose synthetase genes in endosperm and seedling cells of maize: evidence of tissue specific polymerization of promoters. Mol Gen Genet 203: 251–255 (1986).
Chourey, PS, Nelson, OE: The enzymatic deficiency conditioned by the shrunken-1 mutations in maize. Biochem Genet 14: 1041–1055 (1976).
Chourey, PS, Nelson, OE: Interallelic complementation at the sh locus in maize at the enzyme level. Genetics 91: 317–325 (1979).
Heinlein, M, Starlinger, P: Tissue- and cell-specific expression of the two sucrose synthase isoenzymes in developing maize kernels. Mol Gen Genet 215: 441–446 (1989).
Juang, RH, Su, JC: Purification of rice grain sucrose synthase by preparative electrophoresis. J Chin Biochem Soc 17: 42–51 (1988).
Lee, PD, Su, JC: Sucrose-starch transforming system in rice grains — A tracer feeding study. Proc Natl Sci Counc ROC B 6: 189–196 (1982).
Maraña, C, García-Olmedo, F, Carbonero, P: Different expression of two types of sucrose synthase-encoding genes in wheat in response to anaerobiosis, cold shock and light. Gene 88: 167–172 (1990).
McCarty, DR, Shaw, JR, Hannah, LC: The cloning, genetic mapping and expression of the constitutive sucrose synthase locus of maize. Proc Natl Acad Sci USA 83: 9099–9103 (1986).
McElfresh, KC, Chourey, PS: Anaerobiosis induces transcription but not translation of sucrose synthase in maize. Plant Physiol 87: 542–546 (1988).
Rowland, LJ, Chen, YC, Chourey, PS: Anaerobic treatment alters the cell specific expression of Adh-1, Sh, and Sus genes in roots of maize seedlings. Mol Gen Genet 218: 33–40 (1989).
Salanoubat, M, Belliard, G: The steady-state level of potato sucrose synthase mRNA is dependent on wounding, anaerobiosis and sucrose concentration. Gene 84: 181–185 (1989).
Su, JC, Preiss, J: Purification and properties of sucrose synthase from maize kernels. Plant Physiol 61: 389–393 (1978).
Taliercio, EW, Earl, W, Chourey, PS: Post-transcriptional control of sucrose synthase expression in anaerobic seedlings of maize. Plant Physiol 90: 1359–1364 (1989).
Yang, N-S, Russell, D: Maize sucrose synthase-1 promoter directs phloem cell-specific expression of Gus gene in transgenic tobacco plants. Proc Natl Acad Sci USA 87: 4144–4148 (1990).
Yen SF, Sung HY, Su JC: Purification and characterization of rice sucrose synthetase isozymes. Abstract 13-72, 5th Joint Ann Conf Biomed Sci, Taipei (1990).
Zack, CD, Ferl, RJ, Hannah, LC: DNA sequence of a shrunken allele of maize: Evidence for visitation by insertional sequence. Maydica 31: 5–16 (1986).
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Wang, AY., Yu, WP., Juang, RH. et al. Presence of three rice sucrose synthase genes as revealed by cloning and sequencing of cDNA. Plant Mol Biol 18, 1191–1194 (1992). https://doi.org/10.1007/BF00047725
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DOI: https://doi.org/10.1007/BF00047725