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Characterization of cymbidium mosaic virus coat protein gene and its expression in transgenic tobacco plants

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Abstract

Cymbidium mosaic virus (CyMV) is the most prevalent virus infecting orchids. Here, we report the isolation of partial cDNA clones encoding the genomic RNA of CyMV. Like most of the polyadenylated monopartite positive-strand RNA viruses, the open reading frame (ORF) coding for the viral coat protein (CP) is located at the 3′ end. The ORF predicts a polypeptide chain of 220 amino acids with a molecular weight of 23 600. Sequence comparison of this ORF to the CP sequences of potato virus X(PVX) and white clover mosaic virus (WCIMV) revealed a strong amino acid homology in the mid-portion of the CP, but the overall homology was low. The CyMV CP gene was placed downstream of a cauliflower mosaic virus 35S promoter and the chimaeric gene was transferred into Nicotiana benthamiana. Transgenic plants expressing the CyMV CP were protected against CyMV infection.

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Authors and Affiliations

  1. Institute of Molecular and Cell Biology, National University of Singapore, 10 Kent Ridge Crescent, 0511, Singapore, Republic of Singapore

    Tet-Fatt Chia & Yang-Sun Chan

  2. Laboratory of Plant Molecular Biology, The Rockefeller University, 1230 York Avenue, 10021, New York, NY, USA

    Nam-Hai Chua

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  1. Tet-Fatt Chia
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  2. Yang-Sun Chan
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Chia, TF., Chan, YS. & Chua, NH. Characterization of cymbidium mosaic virus coat protein gene and its expression in transgenic tobacco plants. Plant Mol Biol 18, 1091–1099 (1992). https://doi.org/10.1007/BF00047712

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  • DOI: https://doi.org/10.1007/BF00047712

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