Abstract
We have isolated a cDNA clone (pSR132) representing a mRNA which accumulates in senescing carnation flower petals in response to ethylene. In vitro translation of RNA selected by hybridization with pSR132 indicated the mRNA encoded a polypeptide of approximately 36 kDa. This was confirmed by DNA sequence analysis, which predicted a peptide composed of 318 amino acids with a calculated molecular weight of 34.1 kDa. Comparison of the predicted peptide sequence of pSR132 with other proteins compiled in the NBRF data base revealed significant homology with carboxyphosphonoenolpyruvate mutase and phosphoenolpyruvate mutase from Streptomyces hygroscopicus and Tetrahymena pyriformis, respectively. These enzymes are involved in the formation of C-P bonds in the biosynthesis of phosphonates. C-P bonds are found in a wide range of organisms, but their presence or formation in higher plants has not been investigated.
References
Anzai H, Murakami T, Imai S, Satoh A, Nagaoka K, Thompson CJ: Transcriptional regulation of bialaphos biosynthesis in Streptomyces hygroscopicus. J Bact 169: 3482–3488 (1987).
Borochov A, Woodson WR: Physiology and biochemistry of flower petal senescence. Hort Rev 11: 15–43 (1989).
Freeman S, Pollack SJ, Knowles JR: Synthesis of the unusual metabolite carboxy-phosphonoenolpyruvate. Cloning and expression of carboxyphosphonoenolpyruvate mutase. J Am Chem Soc 114: 377–378 (1992).
Hidaka T, Hara O, Satoshi I, Anzai H, Murakami T, Nagaoka K, Seto H: Biochemical mechanism of C-P bond formation of bialaphos: Use of gene manipulation for the analysis of the C-P bond formation step. Agric Biol Chem 54: 2121–2125 (1990).
Hidaka T, Imai S, Hara O, Anzai H, Murakami T, Nagaoka K, Seto H: Carboxylphosphonoenolpyruvate phosphonomutase, a novel enzyme catalyzing C-P bond formation. J Bact 172: 3066–3072 (1990).
Hilderbrand RL, Henderson TO: Phosphonic acids in nature. In: Hilderbrand RL (ed) The Role of Phosphonates in Living Systems, pp. 5–29. CRC Press, Boca Raton FL (1983).
Itzhaki H, Woodson WR: Characterization of an ethylene-responsive glutathione S-transferase gene cluster in carnation. Plant Mol Biol (in press).
Lawton KA, Huang B, Goldsbrough PB, Woodson WR: Molecular cloning and characterization of senescence-related genes from carnation flower petals. Plant Physiol 90: 690–696 (1990).
Lawton KA, Raghothama KG, Goldsbrough PB, Woodson WR: Regulation of senescence-related gene expression in carnation flower petals by ethylene. Plant Physiol 93: 1370–1375 (1990).
Lütcke HA, Chow KC, Mickel FS, Moss KA, Kern HF, Scheele GA: Selection of AUG initiation codons differs in plants and animals. EMBO J 6: 43–48 (1987).
Nichols R: Ethylene production during senescence of flowers. J Hort Sci 41: 279–290 (1966).
Nichols R: The response of carnations (Dianthus caryophyllus) to ethylene. J Hort Sci 43: 335–349 (1968).
Seidel HM, Freeman S, Seto H, Knowles JR: Phosphonate biosynthesis: isolation of the enzyme responsible for the formation of a carbon-phosphorus bond. Nature 335: 457–458 (1988).
Seidel HM, Pompliano DL, Knowles JR: Phosphonate biosynthesis: Molecular cloning of the gene for phosphoenolpyruvate mutase from Tetrahymena pyriformis and overexpression of the gene product in Escherichia coli. Biochemistry 31: 2598–2608 (1992).
Stead AD: Pollination-induced flower senescence: a review. Plant Growth Regul 11: 13–20 (1992).
Wang H, Woodson WR: Reversible inhibition of ethylene action and interruption of petal senescence in carnation flowers by norbornadiene. Plant Physiol 89: 434–438 (1989).
Woodson WR: Changes in protein and mRNA populations during the senescence of carnation petals. Physiol Plant 71: 495–502 (1987).
Woodson WR, Lawton KA: Ethylene-induced gene expression in carnation petals. Relationship to autocatalytic ethylene production and senescence. Plant Physiol 87: 498–503 (1988).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Wang, H., Brandt, A.S. & Woodson, W.R. A flower senescence-related mRNA from carnation encodes a novel protein related to enzymes involved in phosphonate biosynthesis. Plant Mol Biol 22, 719–724 (1993). https://doi.org/10.1007/BF00047414
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00047414