, Volume 39, Issue 3, pp 373–382 | Cite as

The interaction of light intensity and DDT concentration upon the marine diatom, Nitzschia delicatissima cleve

  • Robert B. MacFarlane
  • Walter A. Glooschenko
  • Robert C. Harriss


The effects of p, p′-DDT were determined on photosynthesis and chlorophyll a content in the marine diatom Nitzschia delicatissima Cleve at four light intensities. A consistent reduction in carbon fixation and chlorophyll a per cell over controls in a 24-hour period was observed with increasing DDT concentration between 9.4 ppb and 1000 ppb. The maximum reductions of carbon uptake, chlorophyll a and carbon/chlorophyll a uptake per cell occurred at the highest light intensities. Carbon fixation per cell was reduced by as much as 94% in water containing an initial DDT concentration of 100 ppb, and chlorophyll a per cell by as much as 86% in 220 ppb DDT. Above 100 ppb, further decreases of carbon fixation and chlorophyll a per cell were not observed. Distortion of the chloroplasts in the cells exposed to DDT was also observed at the lowest concentration of DDT used. At 1000 ppb, chloroplasts were totally destroyed within 24 hours.


Chlorophyll Light Intensity Photosynthesis Carbon Fixation High Light Intensity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Die Wirkung von p, p′-DDT auf Photosynthese und Chlorophyll a Gehalt der Meeresdiatomee Nitschia delicatissima Cleve wurde untersucht unter vier Lichtintensitäten. Bei DDT Konzentrationen, steigend von 9,4 ppb bis 1000 ppb, wurde eine fortschreitende Reduktion der Kohlenstoff bindung und des Chlorophyll a Gehaltes pro Zelle gefunden, wobei die höchtse Lichtintensität die stärkste Reduktion, sowie auch die niedrigste Kohlenstoff/Chlorophyll a Aufnahme pro Zelle zeigte. In Wasser mit anfänglichem DDT Gehalt von 100 ppb, wurde die Kohlenstoff bindung pro Zelle 94% reduziert und beim DDT Gehalt von 220 ppb, wurde der Chlorophyll a Gehalt mit 86% reduziert. Bei Konzentrationen über 100 ppb, fielen Kohlenstoff bindung und Chlorophyll a Gehalt pro Zelle nicht weiter ab.

Schädigungen der Chloroplasten in der Zelle wurden schon bei den niedrigsten DDT Gehalten festgestelt. Bei 100 ppb wurden die Chloroplasten innerhalb 24 Stunden völlig zerstört.


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  1. Barthel, W. F., Hawthorne, J. C., Ford, J. H., Bolton, G. C., McDowell, L. L., Grissinger, E. H., & Parsons, D. A. - 1969 - Pesticide residues in sediments of the lower Mississippi River and its tributaries. Pesticides Monitoring J. 3: 8–67.Google Scholar
  2. Croker, R. A. & Wilson, A. S. - 1965 - Kinetics and effects of DDT in a tidal marsh ditch. Trans. Amer. Fish. Soc. 94: 152.Google Scholar
  3. Doty, M. S. & Oguri, M. - 1957 - Evidence for a photosynthetic daily periodicity. Limnol. Oceanogr. 2: 37–40.Google Scholar
  4. Huang, J. & Gloyna, E. F. - 1968 - Effects of organic compounds on photosynthetic oxygenation — I. Chlorophyll destruction and suppression of photosynthetic oxygen production. Water. Res. 2: 347–366.Google Scholar
  5. Lawler, P. D. & Rogers, L. J. 1967 - Effect of DDT on photosynthesis in certain varieties of barley. Nature 215: 1515–1516.Google Scholar
  6. Menzel, D. W., Anderson, J. & Randtke, A. - 1970 - Marine phytoplankton vary in their response to chlorinated hydrocarbons. Science 167: 1724–1726.Google Scholar
  7. Seba, D. B. & Corcoran, E. F. - 1969 - Surface slicks as concentrators of pesticides in the marine environment. Pesticides Monitoring J. 3: 190–193.Google Scholar
  8. Strickland, J. D. H. & Parsons, T. R. - 1968 - A practical handbook of sea water analysis. Fish. Res. Bd. Can. Bull. 167: 331 p.Google Scholar
  9. Weaver, L., Gunnerson, C. G., Breidenbach, A. W. & Lichtenburg, J. J. - 1965- Chlorinated hydrocarbon pesticides in major U. S. river basins. Public Health Rep. 80: 481–493.Google Scholar
  10. Woodwell, G. M., Wurster, C. F. & Isaacson, P. A. - 1967 - DDT residues in an east coast estuary, a case of biological concentration of a persistent pesticide. Science 156: 821–824.Google Scholar
  11. Wurster, C. F. - 1968 - DDT residues photosynthesis by marine phytoplankton. Science 159: 1474–1475.Google Scholar
  12. Yentsch, C. S. & Lee, R. W. - 1966 - A study of photosynthetic light reactions, and a new interpretation of sun and shade phytoplankton. J. mar. Res. 24: 319–337.Google Scholar
  13. Yentsch, C. S. & Ryther, J. H. - 1957 - Short-term variations in phytoplankton chlorophyll and their significance. Limnol. Oceanogr. 2: 140–142.Google Scholar

Copyright information

© Dr. W. Junk b. v. Publishers 1972

Authors and Affiliations

  • Robert B. MacFarlane
    • 1
  • Walter A. Glooschenko
    • 1
  • Robert C. Harriss
    • 1
  1. 1.Department of OceanographyFlorida State UniversityTallahasseeU.S.A.

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