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Genetic analysis of Klebsiella pneumoniae alginate lyase by transposon Tn10 mutagenesis

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References

  • Boyd, J. & J. R. Turvey, 1977. Isolation of a poly α-L-guluronate lyase from Klebsiella aerogenes. Carbohydr. Res. 57: 163–171.

    Google Scholar 

  • Dunne, W. M. jr. & F. L. A. Buckmire, 1985. Partial purification and characterization of a polymannuronic acid depolymerase produced by a mucoid strain of Pseudomonas aeruginosa isolated from a patient with cystic fibrosis. Appl. environ. Microbiol.: 50: 562–576.

    Google Scholar 

  • Favorov, V. V., E. I. Vozhova, V. A. Denisenko & L. A. Elyakova, 1979. A study of the reaction catalyzed by alginate lyase VI from the sea mollusc, Littorina sp. Biochem. Biophys. Acta. 569: 259–266.

    Google Scholar 

  • Foster, T. J., 1984. Analysis of plasmids with transposons. Meth. Microbiol. 17: 197–231.

    Google Scholar 

  • Gunsalus, I. C., 1985. Notes on metabolic plasmid organisation and expression. In D. R. Holinski, S. N. Cohen, D. B. Clewell, D. A. Jackson & A. Hollaender (eds), Plasmids in Bacteria, Plenum Press, New York: 687–706.

    Google Scholar 

  • Hanahan, D., 1985. Techniques for the transformation of E. coli. In D. M. Glover (ed.), DNA Cloning Vol. 1, IRL Press, Oxford, UK: 109–135.

    Google Scholar 

  • Hardy, K. G., 1986. Bacterial Plasmids. Van Nostrand Reinhold, Wokingham, UK, 114 pp.

    Google Scholar 

  • Hashimoto-Gotoh, T., F. C. H. Franklin, A. Nordheim & K. N. Timmis, 1981. Specific purpose plasmid cloning vectors. I. Low copy number, temperature sensitive, mobilization defective pSC101-derived containment vectors. Gene 16: 277–235.

    Google Scholar 

  • Holmes, D. S. & M. Quigley, 1981. A rapid boiling method for the preparation of bacterial plasmids. Anal. Biochem. 114: 193–199.

    Google Scholar 

  • Kado, C. I. & S. T. Liu, 1981. Rapid procedure for detection and isolation of large and small plasmids. J. Bacteriol. 145: 1365–1373.

    Google Scholar 

  • Kleckner, N., 1981. Transposable elements in prokaryotes. Ann. Rev. Genet. 15: 341–404.

    Google Scholar 

  • Maniatis, T., E. F. Fritsch & J. Sambrook, 1982. Molecular Cloning. A Laboratory Manual. Cold Spring Harbor, New York, USA, 545 pp.

    Google Scholar 

  • Madgwick, J., A. Haug & B. Larson, 1978. Ionic requirements of alginate-modifying enzymes in the marine alga Pelvetia canaliculata (L.) Dcne. et Thur. Bot. mar. 11: 1–3.

    Google Scholar 

  • Meynell, E. & N. Datta, 1967. Mutant drug resistant factors of high transmissibility. Nature (Lond.) 214: 885–887.

    Google Scholar 

  • Rochelle, P. A., J. C. Fry, M. J. Day & M. J. Bale, 1985. An accurate method for estimating sizes of small and large plasmids and DNA fragments by gel electrophoresis. J. gen. Microbiol. 132: 53–59.

    Google Scholar 

  • Tseng, C. K., 1984. Phycological research in the development of the Chinese seaweed industry. Proc. int. Seaweed Symp. 11: 7–18.

    Google Scholar 

  • Wainwright, M. & V. Sherbrock-Cox, 1981. Factors influencing alginate degradation by marine fungi: Dendryphiella salina and D. arenaria. Bot. mar. 14: 489–491.

    Google Scholar 

  • Way, J. C., M. A. Davis, D. Morisato, D. E. Roberts & N. Kleckner, 1984. New Tn10 derivatives for transposon mutagenesis and for construction of lacZ operon fusions by transposition. Gene 32: 369–379.

    Google Scholar 

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Gacesa, P., Leaves, G.S. & Weightman, A.J. Genetic analysis of Klebsiella pneumoniae alginate lyase by transposon Tn10 mutagenesis. Hydrobiologia 151, 571–575 (1987). https://doi.org/10.1007/BF00046184

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