Abstract
Anthers and ovaries of Vitis longii ‘Microsperma’ produced embryogenic callus when cultured on solidified Murashige and Skoog medium with 5μM 2,4-dichlorophenoxyacetic acid (2,4-D) and 1μM benzyladenine (BA). The initial callus was short-lived. However, long-term embryogenesis from callus was maintained through serial transfers by careful selection of clustered embryos with subtending callus. Alternatively, long term culture maintenance was through secondary embryogenesis which occurred directly from previously formed embryos on medium lacking growth regulators. Somatic embryos were white, exhibited frequent pluricotyly and tended to be larger than zygotic embryos. Histology of embryogenic callus demonstrated the presence of lipid-like substances and abundant starch. Somatic embryos were attached to callus by narrow to wide suspensor-like structures and possessed typical epidermal, cortical, and vascular tissue. Embryo cells contained abundant lipid-like accumulations but no starch. Embryos germinated when placed on medium containing 1μM BA and produced plants of normal appearance.
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References
Ammirato PV (1983) The regulation of somatic embryo development in plant cell cultures: Suspension culture techniques and hormone requirements. Bio/Technology 1:68–74
dos Santos AVP, Cutter EG, Davey MR (1983) Origin and development of somatic embryos in Medicago sativa L. (Alfalfa). Protoplasma 117:107–115
Gray DJ, Conger BV (1985) Somatic embryo ontogeny in tissue cultures of orchardgrass. In: Henke RR, Hughes KW, Constantin MJ, Hollaender A (eds) Tissue Culture in Forestry and Agriculture. New York: Plenum Press, pp 49–57
Konar RN, Thomas E, Street HE (1972) Origin and structure of embryoids arising from epidermal cells of the stem of Ranunculus sceleratus L. J Cell Sci 11:77–93
Krul WR (1982) In vitro propagation of grape. US Patent 4,532,733, pp 1–8
Krul WD, Worley JF (1977) Formation of adventitious embryos in callus cultures of ‘Seyval’, a French hybrid grape. J Amer Soc Hort Sci 102:360–363
Mullins MG, Rajasekaran K (1980) Plantlets from cultured anthers of grape. In: Proc of the 3rd International Symposium on Grape Breeding, Univ Calif, pp 111–119
Mullins MG, Srinivasan C (1976) Somatic embryos and plantlets from an ancient clone of the grapevine (cv. Cabernet-Sauvignon) by apomixis in vitro. J Exp Bot 27:1022–1030
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco cultures. Physiol Plant 15:473–497
Nabors MW, Heyser JW, Dykes TA, DeMott KJ (1983) Long duration, high-frequency plant regeneration from cereal tissue cultures. Planta 157:385–391
Rajasekaran K, Mullins MG (1979) Embryos and plantlets from cultured anthers of hybrid grapevines. J Exp Bot 30: 399–407
Rajasekaran K, Mullins MG (1983) Influence of genotype and sex-expression on formation of plantlets by cultured anthers of grapevine. Agronomie 3:233–238
Rajasekaran K, Mullins MG (1983) The origin of embryos and plantlets from cultured anthers on hybrid grapevines. Amer J Enol Vitic 34:108–113
Srinivasan C, Mullins MG (1980) High-frequency somatic embryo production from unfertilized ovules of grape. Sci Hort 13:245–252
Zou C, Li P (1981) Induction of pollen plants of grape (Vitis vinifera L.). Acta Bot Sinica 23:79–81
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Gray, D.J., Mortensen, J.A. Initiation and maintenance of long term somatic embryogenesis from anthers and ovaries of Vitis longii ‘Microsperma’. Plant Cell Tiss Organ Cult 9, 73–80 (1987). https://doi.org/10.1007/BF00046081
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DOI: https://doi.org/10.1007/BF00046081