Abstract
Root segments (1 cm long) were excised from 15–20 day old seedlings of silktree (Albizzia julibrissin) grown on B5 medium. About 50% of the control (no growth regulators added) root explants formed shoot buds within 15 days after placement on the culture medium. After 30 days, there were about 4 shoots per control explant. Addition of low levels of various auxins (0.5 μM) did not influence the formation of shoot buds from the explants. Higher concentrations (5μM), however, decreased shoot regeneration. Kinetin and 2iP did not influence shoot regeneration at the concentrations tested (1 & 10 μM). Addition of benzyladenine, Zeatin, or thidiazuron to the culture medium increased both the percentage of explants that formed shoots and the number of shoots per explant. Thidiazuron was highly effective in stimulating shoot formation at low concentrations (<1 μM). At 0.05 μM thidiazuron, 95% of the explants produced shoots and about 10 shoots were formed per explant. Compared to TDZ, higher concentrations (10 μM) of benzyladenine and Zeatin were required to enhance shoot formation. Upon excision and transfer to B5 medium, regenerated shoots developed into normal rooted plantlets.
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Abbreviations
- BA:
-
Benzyladenine
- IAA:
-
Indoleacetic acid
- IBA:
-
Indolebutyric acid
- NAA:
-
Naphthaleneacetic acid
- TDZ:
-
Thidiazuron
- 2ip:
-
Isopentenyladenine
References
Bhat SR, Chitralekha P & Chandel KPS (1992) Regeneration of plants from long-term root culture of lime,Citrus aurantifolia (Christm.) Swing. Plant Cell Tiss. Or. Cult. 29: 19–25
Chaturvedi HC & Sharma M (1987) Excised root culture- A novel method for germplasm conservation. In: Reddy GM (ed) Plant Cell and Tissue Culture of Economically Important Plants (pp 175–179), Osmania Univ., Hyderabad, India
Eapen S & Gill R (1986) Regeneration of plants from cultured root explants of moth bean (Vigna acontifolia L. Jacq. Marechal). Theor. Appl. Genet. 72: 384–387
Gamborg OL, Miller RA & Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Expt. Cell Res. 50: 151–158
Gill R & Saxena PK (1993) Somatic embryogenesis inNicotiana tabacum L.: Induction by thidiazuron of direct embryo differentiation from cultured leaf disks. Plant Cell Rep. 12: 154–159
Huetteman CA & Preece JE (1993) Thidiazuron: A potent cytokinin for woody plant tissue culture. Plant Cell Tiss. Org. Cult. 33: 105–119
Kantharajah AS, Richards GD & Dodd WA (1992) Roots as a source of explants for the successful micropropagation of carambola (Averrhoa carambola L.) Scientia Hortic. 51: 169–177
Laublin G, Saini HS & Cappadocia M (1991)In vitro plant regeneration via somatic embryogenesis from root cultures of some rhizomatous irises. Plant Cell Tiss. Org. Cult. 27: 15–21
Mok MC, Mok DWS, Turner JE & Mujer CV (1987) Biological and biochemical effects of cytokinin-active phenylurea derivatives in tissue culture systems. HortScience 22: 1194–1197
Sankhla D, Davis TD & Sankhla N (1993) Effect of gibberellin biosynthesis inhibitors on shoot regeneration from hypocotyl explants ofAlbizzia julibrissin. Plant Cell Rep. 13: 115–118
Sankhla D, Davis TD & Sankhla N (1994) Thidiazuron-inducedin vitro shoot formation from roots of intact seedlings ofAlbizzia julibrissin. Plant Growth Regul. 14: 267–272
Saxena PK, Malik KA & Gill R (1992) Induction by thidiazuron of somatic embryogenesis in intact seedlings of peanut. Planta 187: 421–424
Thomas JC & Katterman FR (1986) Cytokinin activity induced by thidiazuron. Plant Physiol. 81: 681–683
Tomar UK & Gupta SC (1988)In vitro plant regeneration of leguminous trees (Albizzia spp.) Plant Cell Rep. 7: 385–388
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Sankhla, D., Davis, T.D. & Sankhla, N. In vitro regeneration of silktree (Albizzia julibrissin) from excised roots. Plant Cell Tiss Organ Cult 44, 83–86 (1996). https://doi.org/10.1007/BF00045917
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DOI: https://doi.org/10.1007/BF00045917