Abstract
Shoot cultures were established from seedling shoot tips of Raphanus sativus var. longipinnatus Bailey cv. Gungjung, (Japanese radish) cultured on a Murashige-Skoog medium supplemented with ca. 4.5–135 μM kinetin or N6-benzyladenine. The latter cytokinin supported overall better growth, and 22.2 μM was adopted for maintenance of established cultures. The nitrate: ammonium levels in the medium proved optimal for growth and shoot proliferation and both these parameters were significantly increased by addition of adenine sulfate or sodium phosphate. Rooting of excised shoots was achieved on auxin containing medium. Indole-3-butyric acid (ca. 5 or 10 μM) also enhanced shoot growth. Plants were easily established in soil, appeared morphologically normal, and flowered.
This is a preview of subscription content, log in via an institution to check access.
References
Conger BV (1981) Cloning agricultural plants via in vitro techniques. Boca Raton: CRC Press
Evans DA, Sharp WR, Ammirato PV, Yamada Y (1983) Handbook of plant cell culture, Vol 1. Techniques for Propagation and Breeding. New York: Macmillan
Hisajima S (1982) Microplant propagation through multiple shoot formation from seeds and embryos. In: Fujiwara A (ed) Plant Tissue Culture 1982. Tokyo: Maruzen, pp 141–142
Kay DE (1973) Root crops. TPI crop and product digest no. 2. The Tropical Products Institute, London: HMSO
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Paek, K.Y., Chandler, S.F. & Thorpe, T.A. Micropropagation of Raphanus sativus L. var. longipinnatus (Japanese radish) cv. Gungjung. Plant Cell Tiss Organ Cult 9, 159–165 (1987). https://doi.org/10.1007/BF00044252
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00044252