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In vitro regeneration of plantlets in Fagraea fragrans Roxb. — a tropical tree

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Abstract

Different vegetative parts of Fagraea fragrans Roxb., a valuable timber tree of South East Asia, were used as explants in in vitro studies.

Nodal segments showed the best growth response as numerous adventitious shoots were regenerated in MS medium supplemented with Benzyl adenine (BA, 8.8 um) and 2,4-dichlorophenoxyacetic acid (2,4-D, 0.5 um). Shoot buds also developed from the leaf and root segments that were subcultured. The detailed process of callus growth and differentiation leading to the formation of whole plantlets is described. Uniform plantlets obtained could be transplanted successfully in soil.

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Authors and Affiliations

  1. Botany Department, National University of Singapore, Kent Ridge, 0511, Singapore

    S. K. Lee & A. N. Rao

Authors
  1. S. K. Lee
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  2. A. N. Rao
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Lee, S.K., Rao, A.N. In vitro regeneration of plantlets in Fagraea fragrans Roxb. — a tropical tree. Plant Cell Tiss Organ Cult 7, 43–51 (1986). https://doi.org/10.1007/BF00043920

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  • DOI: https://doi.org/10.1007/BF00043920

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