Abstract
From a pollen tube cDNA library ofPetunia inflata, we isolated cDNA clones encoding a protein, PPE1, which exhibits sequence similarity with plant, bacterial, and fungal pectin esterases. Genomic clones containing thePPE1 gene were isolated using cDNA for PPE1 as a probe, and comparison of the cDNA and genomic sequences revealed the presence of a single intron in thePPE1 gene. During pollen development,PPE1 mRNA was first detected in anthers containing uninucleate microspores; it reached the highest level in mature pollen and persisted at a high level inin vitro germinated pollen tubes. The observed expression pattern of thePPE1 gene suggests that its product may play a role in pollen germination and/or tube growth.
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References
Albani D, Altosaar I, Arnison PG, Fabijanski SF: A gene showing sequence similarity to pectin esterase is especially expressed in developing pollen ofBrassica napus. Sequences in its 5′ flanking region are conserved in other pollen-specific promoters. Plant Mol Biol 16: 501–513 (1991).
Clarke AE, Gleeson PA: Molecular aspects of recognition and response in the pollen-stigma interactions. Rec Adv Phytochem 15: 161–211 (1981).
Coleman CE, Kao T-h: The flanking regions of twoPetunia inflata S alleles are heterogeneous and contain repetitive sequences. Plant Mol Biol 18: 725–737 (1992).
Collmer A, Keen NT. The role of pectic enzymes in plant pathogenesis. Annu Rev Phytopath 24: 383–409 (1986).
Fisher RL. Role of cell wall hydrolysis in fruit ripening. Annu Rev Plant Physiol Plant Mol Biol 42: 675–703 (1991).
Khanh NQ, Ruttkowski E, Leidinger K, Albrecht H, Gottschalk M: Characterization and expression of a genomic pectin methyl esterase-encoding gene inAspergillus niger. Gene 106: 71–77 (1991).
Lee M, Macmillan JD: Mode of action of pectic enzyme. III. site of initial action of tomato pectin esterase on highly esterified pectin. Biochemistry 9: 1930–1934 (1970).
Lutcke HA, Chow KC, Mickel FS, Moss KA, Kern HF, Scheele GA: Selection of AUG initiation codons differs in plants and animals. EMBO J 6: 43–48 (1987).
Mascarenhas JP: Gene activity during pollen development. Annu Rev Plant Physiol Plant Mol Biol 41: 317–338 (1990).
Niogret M-F, Dubald M, Mandaron P, Mache R: Characterization of pollen polygalacturonase encoded by several cDNA clones in maize. Plant Mol Biol 17: 1155–1164 (1991).
Plastow GS: Molecular cloning and nucleotide sequence of the pectin methyl esterase ofErwinia chrysanthemi B374. Mol Microbiol 2: 247–254 (1988).
Pressey R, Reger BJ: Polygalacturonase in pollen from corn and other grasses. Plant Sci 59: 57–62 (1989).
Ray J, Knapp J, Grierson D, Bird C, Schuch W: Identification and sequence determination of cDNA clone for tomato pectin esterase. Eur J Biochem 174: 119–124 (1988).
Recourt K, Ebbelaar MEM, Barbisan P, Laats MM, Wichers HW: EMBL databank X68028 and X68029 (1992).
Rogers HJ, Harvey A, Lonsdale DM: Isolation and characterization of a tobacco gene with homology to pectate lyase which is specifically expressed during microsporogenesis. Plant Mol Biol 20: 493–502 (1992).
von Heijne G: A new method for predicting signal sequence cleavage sites. Nucl Acids Res 14: 4683–4690 (1986).
Wing RA, Yamaguchi J, Larabell SK, Ursin VM, McCormick S: Molecular and genetic characterization of two pollen-expressed genes that have sequence similarity to pectate lyases of the plant pathogenErwinia. Plant Mol Biol 14: 17–28 (1989).
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Mu, JH., Stains, J.P. & Kao, Th. Characterization of a pollen-expressed gene encoding a putative pectin esterase ofPetunia inflata . Plant Mol Biol 25, 539–544 (1994). https://doi.org/10.1007/BF00043881
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DOI: https://doi.org/10.1007/BF00043881