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Plant Cell, Tissue and Organ Culture

, Volume 22, Issue 1, pp 37–41 | Cite as

A new, endosperm-supported callus induction method for wheat (Triticum aestivum L.)

  • Tibor Bartók
  • Ferenc Sági
Article
  • 119 Downloads

Abstract

A new, endosperm-supported callus induetion method was developed using mesocotyls of mature wheat embryos. After seed germination under aseptic condition, most of the germ tissues were cut off and only a few mm of the mesocotyl tissue with the scutellum was used for callus induction. The seeds were placed furrow downwards in 2,4-D solution (6–8 mg l-1). Proliferating callus tissues were already observed on the cut surface of the mesocotyls on the 2nd day after inoculation. On the MS nutrient medium, callus formation from the isolated scutella with attached mesocotyls was negligible even after 6 days. For shoot and root regeneration, the calli produced up to 10 days were removed from the seeds and transferred onto a hormone-free MS medium. As shown by histological methods, the plantlets regenerated via organogenesis.

Key words

callus culture mature embryo mesocotyl organogenesis Tritioum aestivum 

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Copyright information

© Kluwer Academic Publishers 1990

Authors and Affiliations

  • Tibor Bartók
    • 1
  • Ferenc Sági
    • 1
  1. 1.Cereal Research InstituteSzegedHungary

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