Abstract
Glass beads were used as an alternative to agar in the study of the role of polyamines in cold hardiness of white clover. Plantlet growth performance, in vitro hardening and cold stress tolerance were similar on both the agar solidified medium and the liquid medium in glass beads matrix. Glass beads allowed media exchange without plant transfer and an easy monitoring of the uptake of putrescine synthesis inhibitor, 14C difluoromethylornithine from the medium. The matrix is recommended in in vitro studies of whole plant physiology, screening procedures and bioassays where media exchange and/or uniform application of a selection pressure is required. There is also 60% saving on media components and the beads can be re-used after acid wash.
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Abbreviations
- DFMA:
-
difluoremethylarginine
- DFMO:
-
difluoromethylornithine
- MS:
-
Murashige and Skoog salts
- Kcpm:
-
thousand counts per minute
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MacLeod, K., Nowak, J. Glass beads as a solid matrix in in vitro study of the role of polyamines in cold hardiness of white clover. Plant Cell Tiss Organ Cult 22, 113–117 (1990). https://doi.org/10.1007/BF00043686
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DOI: https://doi.org/10.1007/BF00043686