Abstract
Callus and cell suspension cultures were established from root and shoot tips of aseptically-grown seedlings of highly cyanogenic Phaseolus lunatus L. varieties. The content of cyanogenic glucosides in the explanted seedling sections decreased during storage, the derived callus cells were free of cyanogenic glycosides. In spite of the non-existence of cyanogenic glucosides, the cyanogen degrading linamarase, the cyanide detoxifying enzyme β-cyanoalanine synthase and also hydroxynitrile lyase were still present in suspension cultures. The linamarase activity equalled the total β-glucosidase activity, of which up to 80% was found in the culture medium. In contrast the β-cyanoalanine synthase and the hydroxy nitrile lyase were entirely localized in the cell biomass.
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References
Cooke, RD (1978) An enzymatic assay for the total cyanide content of cassava (Manihot esculenta Crantz). J. Sci. Food Agric. 29: 345–352
Frehner, M & Conn, EE (1987) The linamarin β-glucosidase in Costa Rican Lima beans (Phaseolus lunatus L.) is apoplastic. Plant Physiol. 84: 1296–1300
Galoyan, SM, Tolosa, EA, Willhardt, JG & Goryachenkova, EV (1981) Some catalytic properties of β-cyanoalanine synthase from white lupine. Biokhimiya 46: 1855–1862
Hendrickson, HR & Conn, EE (1969) Cyanide metabolism in higher plants IV. Purification and properties of the β-CAS of blue lupine. J. Biol. Chem. 244: 2632–2640
Hösel, W (1981) Glycosylation and glycosidases. In: Conn, EE (Ed) The Biochemistry of the Plants, Vol. 7: Secondary Plant Products (pp 725–753). Academic Press, New York
Hösel, W & Barz, W (1975) β-glucosidases from Cicer arietinum L. Eur. J. Biochem. 57: 607–616
Hösel, W, Berlin, J, Hanzlik, TN & Conn, EE (1985) In vitro biosynthesis of 1-(4-hydroxyphenyl)-2-nitroethane and production of cyanogenic compounds in osmotically stressed cell suspension cultures of Eschscholtzia californica Cham. Planta 166: 176–181
Hösel, W & Nahrstedt, A (1976) Spezifische Glucosidasen für das Cyanglucosid Triglochinin — Reinigung und Charakterisierung von β-Glucosidasen aus Alocasia macrorrhiza Schott. Hoppe Seylers Z. Physiol. Chem. 356: 1265–1275
Hughes, MA & Ayre, L (1976) The production of β-glucosidase in cultured cells of Trifolium repens L. Plant Sci. Letters 7: 271–278
Kojima, M, Poulton, JE, Thayer, SS & Conn, EE (1979) Tissue distributions of dhurrin and of enzymes involved in its metabolism in leaves of Sorghum bicolor. Plant Physiol. 63: 1022–1028
Lambert, JL, Ramasamy, J & Pankstelis, JV (1975) Stable reagents for the colorometric determination of cyanide by modified König reaction. Anal. Chem. 47: 916–918
Langebartels, C & Harms, H (1986) Plant cell suspension cultures as test systems for an ecotoxicologic evaluation of chemicals. Growth inhibition effects and comparison with the metabolic fate in intact plants. Angew. Botanik 60: 113–123
Lieberei, R, Selmar, D & Biehl, B (1985) Metabolization of cyanogenic glucosides in Hevea brasiliensis. Pl. Syst. Evol. 150: 49–63
Lieberei, R, Nahrstedt, A, Selmar, D & Gasparotto, L (1986) The occurrence of lotaustralin in the genus Hevea and changes of HCN-potential in developing organs of Hevea brasiliensis. Phytochem. 25: 1573–1578
Manning, K (1988) Detoxification of cyanide by plants and hormone action. In: Evered, D & Harnett, S (Eds) Cyanide Compounds in Biology (pp 92–104) Wiley & Sons, London
Maurer, HR (1968) Disk-Elektrophorese. Walter de Gruyter & Co., Berlin
Miller, JM & Conn, EE (1980) Metabolism of hydrogen cyanide by higher plants. Plant Physiol. 65: 1199–1202
Murashige, T & Skoog, F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Pourmohseni H (1989) Cyanglucoside in der Epidermis von Sommergerste und ihre Bedeutung für die quantitative Resistenz bzw. Anfälligkeit gegenüber Echtem Mehltau (Erysiphe graminis DC f.sp. hordei Marchal). Doctorthesis, Fachbereich Agrarwissenschaften der Universität Göttingen.
Selmar, D, Lieberei, R, Biehl, B & Voigt, J (1987) Hevea linamarase—a nonspecific β-glycosidase. Plant Physiol. 83: 557–563
Selmar, D, Lieberei, R, Biehl, B & Conn, EE (1989) Hydroxynitrile lyase in Hevea brasiliensis and its significance for rapid cyanogenesis. Physiol. Plant. 75: 97–101
Thayer, SS & Conn, EE (1981) Subcellular localization of Dhurrin β-glucosidase and hydroxynitrile-lyase in the mesophyll cells of Sorghum leaf blades. Plant Physiol. 67: 617–622
Till, J (1987) Variability of expression of cyanogenesis in white clover (Trifolium repens L.). Heredity 59: pp 265–271
Wiermann, R (1981) Secondary plant products and cell and tissue differentiation. In: Conn, EE (Ed) The Biochemistry of Plants, Vol. 7: Secondary Plant Products (pp 85–116). Academic Press, New York
Wurtele, ES, Basil, JN & Conn, EE (1985) Subcellular and developmental distribution of β-cyanoalanine synthase in barley leaves. Plant Physiol. 78: 285–290
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~Botanical Institute, Technical University Braunschweig
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Istock, U., Lieberei, R. & Harms, H. Pattern of enzymes involved in cyanogenesis and HCN metabolism in cell cultures of Phaseolus lunatus L. varieties. Plant Cell Tiss Organ Cult 22, 105–112 (1990). https://doi.org/10.1007/BF00043685
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DOI: https://doi.org/10.1007/BF00043685