Skip to main content
SpringerLink
Log in

Somatic embryogenesis from styles of lemon (Citrus limon)

  • Research Note
  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

Lemon [Citrus limon (L.) Burm.] styles were treated with different growth regulators for induction of somatic embryos. Styles and stigmas were dissected from flowers and cultured on a Murashige and Skoog (MS) basal medium supplemented with 4.52 μM 2,4-dichlorophenoxyacetic acid and 13.3 μM 6-benzyladenine. Callus was induced from the style base 2 weeks after the treatment initiation, and embryos appeared 2 months later.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

BA:

6-benzyladenine

2,4-d :

2,4-dichlorophenoxyacetic acid

MS:

Murashige & Skoog (1962)

NAA:

α-naphthaleneacetic acid

References

  • Grosser, JW & Gmitter, FG (1990) Protoplast fusion and citrus improvement. Plant Breed. Rev. 8: 339–374

    Google Scholar 

  • Litz, RE, Moore, GA & Srinivasan, C (1985) In vitro system for propagation and improvement of tropical fruits and palm. Hort. Rev. 7: 157–200

    Google Scholar 

  • Moore, GA (1986) In vitro propagation of Citrus Rootstocks. HortScience 21: 300–301

    Google Scholar 

  • Murashige, T & Skoog, F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant. 15: 473–497

    Google Scholar 

  • Nito, N & Iwamasa, M (1990) In vitro plantlet formation from juice vesicle callus of satsuma (Citrus unshiu Marc.). Plant Cell Tissue Organ Culture 20: 137–140

    Google Scholar 

  • Ohgawara, T, Kobayashi, S, Ishii, S, Yoshinaga, K & Oiyama, I (1989) Somatic hybridization in Citrus: navel orange (C. sinensis Osb.) and grapefruit (C. paradisi Macf.). Theor. Appl. Genet. 78: 609–612

    Google Scholar 

  • Steel, RDG & Torrie, JH (1980) Principles and Procedures of Statistics. 2nd Ed. McGraw-Hill, New York

    Google Scholar 

  • Tisserat, B, Galletta, PD & Jones, D (1989) Induction of adventitious branches from cultured Citrus juice vesicles—A potential means of proliferation. Amer. J. Bot. 76: 1750–1758

    Google Scholar 

  • Tusa, N, Grosser, JW & Gmitter, FG (1990) Plant regeneration of ‘Valencia’ sweet orange, ‘Femminello’ lemon, and the interspecific somatic hybrid following protoplast fusion. J. Amer. Soc. Hort. Sci. 115: 1043–1046

    Google Scholar 

  • Vardi, A, Spiegel-Roy, P & Galun, E (1982) Plant regeneration from citrus protoplasts: Variability in methodological requirements among cultivars and species. Theor. Appl. Genet. 62: 171–176

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Centro di Studio per il Miglioramento Genetico degli Agrumi, Consiglio Nazionale delle Ricerche c/o Facoltà di Agraria, Viale delle Scienze, I-90128, Palermo, Italy

    Francesco Carimi, Fabio De Pasquale & Francesco Giulio Crescimanno

Authors
  1. Francesco Carimi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Fabio De Pasquale
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Francesco Giulio Crescimanno
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Carimi, F., De Pasquale, F. & Crescimanno, F.G. Somatic embryogenesis from styles of lemon (Citrus limon). Plant Cell Tiss Organ Cult 37, 209–211 (1994). https://doi.org/10.1007/BF00043619

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00043619

Key words

Search

Navigation