Abstract
Sophora toromiro (Phil.) Skottsb. is a woody species in imminent danger of extinetion. Since natural regeneration by seeds is poor and plant growth is very slow, asexual propagation is necessary. In vitro regeneration from 3- to 4-month-old seedlings was achieved. A range of naphthaleneacetic acid and benzyladenine concentrations induced root formation in nodal segment explants, developing plantlets, and also promoted axillary bud development. In subculture, nodal sections derived from axillary growth initiated multiple shoot formation and roots in a liquid medium leading to plantlet formation.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- NAA:
-
naphthaleneacetic acid
- BA:
-
benzyladenine
- IBA:
-
indolebutyric acid
- GA3 :
-
gibberellic acid
References
Alden, B & Zizka, G (1989) Der Toromiro (Sophora toromiro)-eine ausgestorbene Pflanze wird wiederentdeckt. Natur und Museum 119: 145–152
Brown, CL & Sommer, HE (1982) Vegetative propagation of dicotyledonous trees. In: JMBonga & DJDurzan (Eds) Tissue Culture in Forestry (pp 119–122). Martinus Nijhoff/Dr. W. Junk, The Hague
Furuya, T & Ikuta, A (1968) The presence of I-maackiain and pterocarpin in callus tissue of Sophora angustifolia. Chem. Pharm. Bull. 16: 771
Lobin, W & Barthlott, W (1988) Sophora toromiro (Leguminosae); the lost tree of Easter Island. Bot. Gardens Conservation News 1: 32–34
Murashige, T & Skoog, F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Nitsch, JP & Nitsch, C (1969) Haploid plants from pollen grains. Science 163: 85–87
Philippi, RA (1873) Bemerkungen über die chilenischen Arten von Edwardsia toromiro. Bot. Zeitung 31: 741–744
Polhill, RM (1981) Papilionoideae. In: Polhill, RM & Raven, P (Eds) Advances in Legume Systematics (pp 191–208). Royal Botanic Gardens, Kew
Rai, PP, Turne, TD & Greenmith, SL (1974) Anthracene derivatives in tissue culture of Cassia senna L. J. Pharm. Pharmc. 26: 722–726
Rao, AN & Lee, SK (1982) Importance of tissue culture in tree propagation. In: Fujiwara, A (Ed) Proc. 5th Intl. Cong. Plant Tissue & Cell Culture (pp 715–718). Maruzen Co., Tokyo
Rodriguez, R, Matthei, O & Quezada, M (1983) Flora Arbórea de Chile. Ediciones Universidad de Concepción, Concepción
Yagi, A, Hiroyuki, H, Nobuyuki, O & Kensuke, H (1992) Production of I-maackiain glucosides in callus tissue of Sophora flavescens. Shoyakugaku Zasshi 46: 298–301
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Iturriaga, L., Jordan, M., Roveraro, C. et al. In vitro culture of Sophora toromiro (Papilionaceae), an endangered species. Plant Cell Tiss Organ Cult 37, 201–204 (1994). https://doi.org/10.1007/BF00043617
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00043617