Abstract
Hieracium aurantiacum L. is being investigated as a model system for studying the genetic basis of gametophytic apomixis. Micropropagation methods were developed for the multiplication and maintenance of unique genotypes, and to facilitate the development of an Agrobacterium-mediated transformation procedure. A basal medium containing Murashige & Skoog macro- and micro-salts, B5 vitamins and 3% sucrose was used throughout. Shoot regeneration from leaf disks was optimized on a medium supplemented with 2.5 μM indole-3-butyric acid (IBA) and 2.2 μM 6-benzyladenine (BA). Shoot proliferation was optimized using 2.2 μM BA, without additional IBA, giving 5-fold shoot-tip multiplication rates every 4 weeks. Shoots readily formed roots on the basal medium and could be transplanted to potting medium 3 weeks later. Micropropagation was found to have no effect on the expression of apomixis in this species.
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Abbreviations
- Ba:
-
6-benzyladenine
- iba:
-
indole-3-butyric acid
- lsd:
-
least significant difference
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Bicknell, R.A. Micropropagation of Hieracium aurantiacum . Plant Cell Tiss Organ Cult 37, 197–199 (1994). https://doi.org/10.1007/BF00043616
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DOI: https://doi.org/10.1007/BF00043616