Abstract
The anther culture response of 18 Hordeum spontaneum derived winter barley lines, as well as that of the F2 and/or BC1 progeny of the cross between nine of these lines and the winter barley cultivar Triton was assessed. While the majority of lines showed a poor regeneration capacity (4%), two lines, RS 170-A13 × Sonja and RS 120-27 × Sonja demonstrated an outstanding response, producing 26 and 8 green regenerants per 100 anthers plated, respectively. Five further lines, RS 70-30 × Dura, RS L74-11 × Dura, RS 122-19 × Sonja. RS 142-29 × Dura and RS 170-A51 × Sonja were identified as highly responsive in the F2 generation. An average production of 4 green regenerants per 100 anthers plated was observed both in the parental and F2 generation.
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Abbreviations
- BA:
-
benzyladenine
- IAA:
-
indole-3-acetic acid
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Kintzios, S., Fischbeck, G. Anther culture response of Hordeum spontaneum-derived winter barley lines. Plant Cell Tiss Organ Cult 37, 165–170 (1994). https://doi.org/10.1007/BF00043611
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DOI: https://doi.org/10.1007/BF00043611