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Production and characterization of intergeneric somatic hybrids through protoplast electrofusion between potato (Solanum tuberosum) and Lycopersicon pennellii

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Abstract

Mesophyll protoplasts of Lycopersicon pennelli Corr., a wild relative of tomato, were electrofused with those from a dihaploid potato clone, cv Nicola, with the objectives of transferring saline tolerance from L. pennellii to cultivated potato. 150 calli were selected from the fusion experiments, finally giving 2 hybrid shoots. Their hybrid nature was verified by examining isoenzyme patterns for esterases (EST), peroxidase (PRX), phosphogluconate dehydrogenase (6-PGD), and glutamate oxaloacetate transaminase (GOT). The hybrid plants had an intermediate morphology, and grew vigorously in vitro. When transplanted to soil, they were less vigorous, due to difficulties in rooting, but were still capable of flowering, and forming short stolons and mishaped tubers, probably resulting from the effects of gene dosage due to the novel association of two genomes from a tuberizing (potato) and a non tuberizing species (L. pennellii). The characteristics of such mishaped tubers provided strong evidence of a hybrid nature for the selected plants. The hybrid plants were highly sterile, producing only 3–7% viable pollen. Tests for salt tolerance showed that the growth of the somatic hybrid plants was reduced by 50% as for L. pennellii, whilst potato did not grow at all under saline conditions.

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Abbreviations

MS:

Murashige and Skoog basal medium

2,4-d :

2,4-dichlorophenoxyacetic acid

NAA:

α-naphthaleneacetic acid

IAA:

indole-3-acetic acid

BAP:

6-benzylaminopurine

PEG:

polyethylen glycol 6,000

MES:

2-(N-morpholino)ethanesulfonic acid

AC:

alternating current

EST:

esterases

PRX:

peroxidase

6-PGD:

phosphogluconate dehydrogenase

GOT:

glutamate oxaloacetate transaminase

FDA:

fluorescein diacetate

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Authors and Affiliations

  1. Morphogénèse Végétale Expérimentale, Bât. 360, Université Paris Sud, 91405, Orsay Cedex, France

    I. Sherraf, S. Tizroutine, M. H. Chaput, M. Allot, I. Mussio, D. Sihachakr, L. Rossignol & G. Ducreux

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  1. I. Sherraf
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  2. S. Tizroutine
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  3. M. H. Chaput
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  4. M. Allot
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  5. I. Mussio
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  6. D. Sihachakr
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  7. L. Rossignol
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  8. G. Ducreux
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Sherraf, I., Tizroutine, S., Chaput, M.H. et al. Production and characterization of intergeneric somatic hybrids through protoplast electrofusion between potato (Solanum tuberosum) and Lycopersicon pennellii . Plant Cell Tiss Organ Cult 37, 137–144 (1994). https://doi.org/10.1007/BF00043607

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  • DOI: https://doi.org/10.1007/BF00043607

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