Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 31, Issue 1, pp 75–79 | Cite as

Reduction of contamination in bulb-explant cultures ofNarcissus by a hot-water treatment of parent bulbs

  • G. M. G. M. Hol
  • P. C. G. van der Linde
Article

Abstract

After disnfestation of explants with 1% sodium hypochlorite for 30 min, 40–60% of twin-scale cultures ofNarcissus ‘Golden Harvest’ remained contaminated, mainly byFusarium spp. Extension of the disinfestation time in hypochlorite from 30 min to 2h or a double disinfestation treatment did not reduce the contamination, suggesting that the fungus may be located inside the tissue. Hot-water treatment of bulbs prior to treatment with sodium hypochlorite resulted in contamination being reduced to 5%, while the regeneration was not affected. Similar results were obtained when experiments were repeated with five other cultivars ofNarcissus.

Key words

Amaryllidaceae Daffodil flowerbulb Fusarium regeneration 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bergman BHH (1962) Warm waterbehandeling van narcissen en Fusariumbolrot. Bloembollencultuur 73 (7): 144–150Google Scholar
  2. Blake J (1987) Mites and thrips as bacterial and fungal vectors between plant tissue cultures. Acta Hort. 225: 163–166Google Scholar
  3. Cassells AC (Ed.) (1987) Bacterial and bacteria-like contaminants of plant tissue cultures. Acta Hort. 225, Intl. Soc. Hort. Sci., WageningenGoogle Scholar
  4. Chow YN (1990) Micropropagation of Narcissus. Ph. D. Thesis. Queen's University of Belfast, Northern IrelandGoogle Scholar
  5. Cohen D & Whitehead HCM (1987) Micropropagation of high-health narcissus and iris. Commercial Rep. 14: 1–29, Plant Physiology Division DSIR, Palmerston North, New ZealandGoogle Scholar
  6. Hawker LE (1935) Further experiments on theFusarium bulb rot of narcissus. Ann. Appl. Biol. 22: 687–708CrossRefGoogle Scholar
  7. Hussey G (1982)In vitro propagation of narcissus. Ann. Bot. 49: 707–719CrossRefGoogle Scholar
  8. Jensen JL (1888) The propagation and prevention of smut in oats and balrey. J Roy. Agric. Soc. Ser. 2, 24: 397–415Google Scholar
  9. Kunneman BPAM & Albers MRJ (1989) Snelheid grootste struikelblok; weefselkweek pioen gaat niet over rozen. Bloembollencultuur 100 (23): 16–17Google Scholar
  10. Leggatt IV & Waites WM (1987) Characterisation of micro-organism isolated from plants during micropropagation. Acta Hort. 225: 93–102Google Scholar
  11. Muller PJ (1966) Voorkomen en bestrijdingen van bladaaltjes bij lelies. Meded Rijksfacult Landb. Wetensch. Gent 31: 666–671Google Scholar
  12. Muller PJ (1989) Warmwaterbehandeling tegen destructoraaltje in tulp: Goed, goedkoop en milieuvriendelijk. Vakwerk 63 (1989) 25: 8–9Google Scholar
  13. Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant. 15: 473–497CrossRefGoogle Scholar
  14. Saaltink GJ & Kamerman W (1971) Hyacinth yellow disease no 34 (16 p). Praktijk meded. Lab. voor Bloembollenonderzoek, LisseGoogle Scholar
  15. Schenk PK (1961) Hot-water treatment of gladiolus cormels. Meded. Dir. Tuinb. 24: 173–185 Landbouw PublicatieGoogle Scholar
  16. Seabrook JEA (1990) Narcissus (Daffodil). In: Ammirato PV, Evans DA, Sharp WR & Bajaj YPS (Eds) Handbook of Plant Cell Culture V: Ornamental Species (pp 577–597). McGraw-Hill Publishing Company, New YorkGoogle Scholar
  17. Seabrook JEA, Cuming BG & Dionne L (1976) Thein vitro induction of adventitious shoot and root apices onNarcissus (daffodil and narcissus) cultivar tissue. Can. J. Bot. 54: 814–819CrossRefGoogle Scholar
  18. Van Slogteren E (1925) De toepassing van warmte bij de bestrijding van bloembollenziekten en den invloed hiervan op den bloei der gewassen. Handelingen van het XXe Ned. natuur-en Geneesk. Congres 1925Google Scholar
  19. Van Slogteren E (1958) Hot-water treatment. Daffodil and Tulip Yearbook 1958: 30–34Google Scholar
  20. Vreeburg PJM (1981a) De grote narcisvlieg en haar bestrijding. Bloembollencultuur 91 (39): 1059Google Scholar
  21. Vreeburg PJM (1981b) De narcismijt en haar bestijding. Bloembollencultuur 92 (6): 154–155Google Scholar

Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • G. M. G. M. Hol
    • 1
    • 2
  • P. C. G. van der Linde
    • 1
    • 2
  1. 1.Bulb Research CentreLisseThe Netherlands
  2. 2.Central Research Laboratory for Tissue Culture of Horticultural CropsLisseThe Netherlands

Personalised recommendations