Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 31, Issue 1, pp 75–79 | Cite as

Reduction of contamination in bulb-explant cultures ofNarcissus by a hot-water treatment of parent bulbs

  • G. M. G. M. Hol
  • P. C. G. van der Linde


After disnfestation of explants with 1% sodium hypochlorite for 30 min, 40–60% of twin-scale cultures ofNarcissus ‘Golden Harvest’ remained contaminated, mainly byFusarium spp. Extension of the disinfestation time in hypochlorite from 30 min to 2h or a double disinfestation treatment did not reduce the contamination, suggesting that the fungus may be located inside the tissue. Hot-water treatment of bulbs prior to treatment with sodium hypochlorite resulted in contamination being reduced to 5%, while the regeneration was not affected. Similar results were obtained when experiments were repeated with five other cultivars ofNarcissus.

Key words

Amaryllidaceae Daffodil flowerbulb Fusarium regeneration 


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Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • G. M. G. M. Hol
    • 1
    • 2
  • P. C. G. van der Linde
    • 1
    • 2
  1. 1.Bulb Research CentreLisseThe Netherlands
  2. 2.Central Research Laboratory for Tissue Culture of Horticultural CropsLisseThe Netherlands

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