Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 31, Issue 1, pp 47–50 | Cite as

Somatic embryogenesis and high frequency plantlet regeneration in callus cultures ofThevetia peruviana

  • Anjani Kumar
Article

Abstract

Plantlet regeneration through somatic embryogenesis has been achieved in the apocynaceous medicinal treeThevetia peruviana L. Calluses obtained by culturing young leaf discs on MS medium containing 9 μM 2,4-dichlorophenoxyacetic acid and 4.6 μM kinetin, when subjected to reduced levels of the growth regulators followed by higher cytokinin treatment, produced numerous somatic embryos. Somatic embryos developed into complete plantlets on a medium devoid of growth regulators. An average of 40–50 plantlets were obtained from 50 mg of embryogenic callus. Survival of transplants was 60% under glasshouse conditions.

Key words

embryoids leaf callus medicinal tree 

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References

  1. Backs-Husemann D & Reinert J (1970) Embryos formation by isolated single cells from tissue culture ofDaucus carota. Protoplasma 70: 49–60CrossRefGoogle Scholar
  2. Bhakuni DS (1990) Drugs from plants. Sci. Rep. 8: 12–17Google Scholar
  3. Dasgupta M & Datta SK (1987) Tissue culture, cytology and pharmacognostic evaluation ofThevetia peruviana. In: Chauhan YS (Ed) Biology, Cultivation and Utilization of Some Medicinal and Aromatic Plants (pp 49–57), Meghalaya Science Society, ShillongGoogle Scholar
  4. Eapen S & George L (1989) High frequency plant regeneration through somatic embryogenesis in finger millet (Eleusine caracana Gaertn.). Plant Sci. 61: 127–130CrossRefGoogle Scholar
  5. Guimaraes MLS, Cruz GS & Montezuma-de-Carvalho JM (1988) Somatic embryogenesis and plant regeneration inCyphomandra betacea (Cav.) Sendt. Plant Cell Tiss. Org. Cult. 15: 161–167CrossRefGoogle Scholar
  6. Haccius B (1978) Question of unicellular origin of nonzygotic embryos in callus culture. Phytomorphology 28: 74–81Google Scholar
  7. Halperin W (1969) Morphogenesis in cell cultures. Annu. Rev. Plant Physiol. 21: 395–418CrossRefGoogle Scholar
  8. Konar RN, Thomas E & Street HE (1972) Origin and structure of embryoids arising from epidermal cells of the stem ofRanunculus scleratus L. J. Cell Sci. 11: 77–93PubMedGoogle Scholar
  9. Larkin P J & Scowcroft WR (1981) Somaclonal variation — a novel source of variability from cell cultures for plant improvement. Theor. Appl. Genet. 60: 197–214CrossRefGoogle Scholar
  10. Mariotti D & Arcioni S (1983) Callus culture ofCoronilia varia L. (Crownvetch): plant regeneration through somatic embryogenesis. Plant Cell Tiss. Org. Cult. 2: 103–110CrossRefGoogle Scholar
  11. McWilliam AA, Smith SM & Street HE (1974) The origin and development of embryoids in suspension cultures of carrot. Ann. Bot. 38: 243–250CrossRefGoogle Scholar
  12. Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497CrossRefGoogle Scholar
  13. Pierson ES, Van Lemmeren AAM, Schel THN & Staritsky G (1983)In vitro development of embryoids from punched leaf discs ofCoffea canephora. Protoplasma 115: 208–216CrossRefGoogle Scholar
  14. Scowcroft WR (1977) Somatic cell genetics and plant improvement. Adv. Agron. 29: 39–81CrossRefGoogle Scholar
  15. Thomas E, King PJ & Potrykus I (1979) Improvement of crop plants via single cellin vitro: an assessment. Z. Pflanzenphysiol. 82: 1–30Google Scholar
  16. von Arnold S & Wallin A (1988) Tissue culture methods for clonal propagation of forest trees. Intl. Assoc. Plant Tissue Culture Newsl. 56: 2–13Google Scholar

Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • Anjani Kumar
    • 1
  1. 1.Department of BotanyNorth-Eastern Hill UniversityShillongIndia

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