Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 31, Issue 1, pp 15–19 | Cite as

Micropropagation of a recalcitrant male asparagus clone (MD 22-8)

  • R. D. Cheetham
  • C. Mikloiche
  • M. Glubiak
  • P. Weathers
Article
  • 55 Downloads

Abstract

TheAsparagus officinalis male asparagus clone MD 22–8 (Howard Scott) is highly prized by asparagus breeders but has been very difficult to micropropagate due to its slow growth rate and reluctance to initiate roots. Shoot tips cultured on indole-3-acetic acid or indole-3-propionic acid, at concentrations of 1.53 and 1.44 μM respectively, resulted in levels of root initiation and elongation equal to or better than those of a female asparagus clone. These culture conditions also improved the rates of shoot initiation and elongation. Root initiation and elongation were further increased by culture conditions that provided enhanced gas exchange.

Key words

auxins gas exchange root and shoot initiation 

Abbreviations

BA

6-benzyladenine

IAA

3-indoleacetic acid

IPA

indolepropionic acid

NAA

α-naphthaleneacetic acid

2,4-d

2,4-dichlorophenoxyacetic acid

2,4,5-T

2,4,5-trichlorophenox-yacetic acid

PPF

photosynthetic photon flux

MS

Murashige & Skoog

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Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • R. D. Cheetham
    • 1
  • C. Mikloiche
    • 1
  • M. Glubiak
    • 1
  • P. Weathers
    • 1
  1. 1.Department of Biology and BiotechnologyWorcester Polytechnic InstituteWorcesterUSA

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