Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 31, Issue 1, pp 15–19 | Cite as

Micropropagation of a recalcitrant male asparagus clone (MD 22-8)

  • R. D. Cheetham
  • C. Mikloiche
  • M. Glubiak
  • P. Weathers


TheAsparagus officinalis male asparagus clone MD 22–8 (Howard Scott) is highly prized by asparagus breeders but has been very difficult to micropropagate due to its slow growth rate and reluctance to initiate roots. Shoot tips cultured on indole-3-acetic acid or indole-3-propionic acid, at concentrations of 1.53 and 1.44 μM respectively, resulted in levels of root initiation and elongation equal to or better than those of a female asparagus clone. These culture conditions also improved the rates of shoot initiation and elongation. Root initiation and elongation were further increased by culture conditions that provided enhanced gas exchange.

Key words

auxins gas exchange root and shoot initiation 





3-indoleacetic acid


indolepropionic acid


α-naphthaleneacetic acid


2,4-dichlorophenoxyacetic acid


2,4,5-trichlorophenox-yacetic acid


photosynthetic photon flux


Murashige & Skoog


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Cheetham RD, Weathers P, DiIorio A, Glubiak M & Mikloiche C (1990)In vitro growth of a recalcitrant male asparagus cultivar. Abstracts VII Intl. Congress on Plant Tissue and Cell Culture. Amsterdam, The Netherlands, 24–29 June (p. 94)Google Scholar
  2. Chin C (1982) Promotion of shoot and root formation in asparagusin vitro by ancymidol. HortScience 17: 590–591Google Scholar
  3. Desjardins Y, Tiessen H & Harney PM (1987) The effect of sucrose and ancymidol on thein vitro rooting of nodal sections of asparagus. HortScience 22: 132–133Google Scholar
  4. Doré C (1977)In vitro techniques as an efficient tool in asparagus breeding. Acta Hort. 78: 89–93CrossRefGoogle Scholar
  5. Doré C (1988) Nouveau regard sur le comportement de l'asperge (Asparagus officinalis L.) multipliéein vitro. Agronomie 8: 843–850CrossRefGoogle Scholar
  6. Doré C (1990) Asparagus cloning revisited. Acta Hort. 271: 101–108CrossRefGoogle Scholar
  7. Ellison JH & Kinelski JJ (1986) Cultivar Greenwich a male asparagusAsparagus officinalis hybrid. HortScience 21: 1249Google Scholar
  8. Fox JL (1988) Plants thrive in ultrasonic nutrient mists. Biotechnology 6: 361Google Scholar
  9. Hasegawa PM, Murashige T & Takatori FH (1973) Promotion of asparagus through shoot apex culture II. Light and temperature requirements, transplantability of plants, and cytohistological characteristics. J. Amer. Soc. Hort. Sci. 98: 143–148Google Scholar
  10. Jackson MB, Abbott AJ, Belcher AR, Hall KC, Butler R & Cameron J (1991) Ventilation in plant tissue cultures and effects of poor aeration on ethylene and carbon dioxide accumulation, oxygen depletion and explant development. Ann. Bot. 67: 229–237CrossRefGoogle Scholar
  11. Kar DK & Sen S (1985) Propagation ofAsparagus racemousus through tissue culture. Plant Cell Tiss. Org. Cult. 5: 89–95CrossRefGoogle Scholar
  12. Khunachak A, Chin C, Le T & Gianfagna T (1987) Promotion of asparagus shoot and root growth by growth retardants. Plant Cell Tiss. Org. Cult. 11: 97–110CrossRefGoogle Scholar
  13. Kong Y & Chin C (1988) Culture of asparagus protoplasts on porous polypropylene membrane. Plant Cell Rep. 7: 67–69CrossRefPubMedGoogle Scholar
  14. Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497CrossRefGoogle Scholar
  15. Murashige T, Shabde MN, Hasegawa PM, Takatori FH & Jones JB (1972) Propagation of asparagus through shoot apex culture I. Nutrient medium for formation of plantlets. J. Amer. Soc. Hort. Sci. 97: 158–161Google Scholar
  16. Rossi V & DePaoli G (1986) Micropropagatione e conservazionein vitro del asparago. Agricoltura Ricerca 63–64: 33–40Google Scholar
  17. Volokita M, Levi A & Sink KC (1987) A revised protocol for micropropagation of asparagus. Asparagus Research Newsletter. (pp 8–17). Mich. State Univ., E. Lansing, MIGoogle Scholar
  18. Yang H (1977) Tissue culture technique for asparagus propagation. HortScience 12: 16–17Google Scholar
  19. Yang HJ & Clore WJ (1973) Rapid vegetative propagation of asparagus through lateral bud culture. HortScience 8: 141–143Google Scholar
  20. Yang HJ & Clore WJ (1974) Development of complete plantlets from modraately vigorous shoots of stock plants of asparagusin vitro. HortScience 9: 138–143Google Scholar

Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • R. D. Cheetham
    • 1
  • C. Mikloiche
    • 1
  • M. Glubiak
    • 1
  • P. Weathers
    • 1
  1. 1.Department of Biology and BiotechnologyWorcester Polytechnic InstituteWorcesterUSA

Personalised recommendations