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Influence of tissue culture conditions on apogamy in Dryopteris affinis sp. affinis

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Abstract

The naturally-occurring apogamy of some ferns can be modified by culture conditions and growth regulators. Gametophytes of the apogamic fern Dryopteris affinis sp. affinis L., were cultured on Murashige and Skoog (MS) basal medium. Changes in concentration of MS medium components, sucrose, agar and different pH values were tested. The addition of benzyladenine (4.43 μM) and naphthalene acetic acid (0.53 μM) enhanced sporophyte proliferation on the gametophytes. After one month in culture, the gametophytes formed callus with a high morphogenic capacity. Culture of calli on medium without growth regulators yielded about 10,000 sporophytes per 1 g fresh weight of callus. This pattern of differentiation slowed with time to a point where only gametophyte regeneration was observed.

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Abbreviations

BA:

benzyladenine

2,4-d :

2,4-dichlorophenoxyacetic acid

F.W.:

fresh weight

MS:

Murashige & Skoog medium

NAA:

1-naphthalene acetic acid

SE:

standard error

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Authors and Affiliations

  1. Department B. O. S. Lab. Fisiologia Vegetal, University of Oviedo, Catedrático Rodrigo Uría s/n, 33071, Oviedo, Spain

    H. Fernandez, A. M. Bertrand & R. Sanchez-Tames

Authors
  1. H. Fernandez
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  2. A. M. Bertrand
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  3. R. Sanchez-Tames
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Fernandez, H., Bertrand, A.M. & Sanchez-Tames, R. Influence of tissue culture conditions on apogamy in Dryopteris affinis sp. affinis . Plant Cell Tiss Organ Cult 45, 93–97 (1996). https://doi.org/10.1007/BF00043434

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  • DOI: https://doi.org/10.1007/BF00043434

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