Abstract
In vitro plantlet regeneration systems for the seed geranium (Pelargonium x hortorum Bailey) using cotyledon, hypocotyl and root explants were optimized by studying the influence of seedling age, growth regulators and excision orientation on organogenesis. Indole-3-acetic acid combined with zeatin yielded the highest rate of shoot production on cotyledon explants (0.2–2 shoots per explant). More shoots were produced on explants cut from the most basal region of cotyledons from 2 to 4-day-old seedlings than from older seedlings or more distal cut sites. Hypocotyl explants produced the highest number of shoots, up to 40 shoots per explant, on indole-3-acetic acid (2.8–5.6 mM) + zeatin (4.6 mM) or thidiazuron (4.5 mM). Maximum shoot formation (0.3–1.4 shoots per explant) on root explants occurred when they were cultured on medium containing zeatin. Regenerated shoots rooted best on a basal medium containing no growth regulators. There were substantial differences among cultivars in shoot formation from each of the explant systems.
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Abbreviations
- BA:
-
6-benzylaminopurine
- 2,4-d :
-
2,4-dichlorophenoxyacetic acid
- IAA:
-
indole-3-acetic acid
- NAA:
-
naphthaleneacetic acid
- TDZ:
-
thidiazuron
References
Arroyo R & Revilla M (1991) In vitro plant regeneration from cotyledon and hypocotyl segments in two bell pepper cultivars. Plant Cell Rep 10: 414–416
Chraibi B, Castelle J-C, Latche A, Roustan J-P & Fallot J (1992) Enhancement of shoot regeneration potential by liquid medium culture from mature cotyledons of sunflower (Helianthus annuus L.). Plant Cell Rep. 10: 617–620
Debergh H & Maene L (1977) Rapid clonal propagation of pathogen-free pelargonium plants starting from shoot tips and apical meristems. Acta Hort. 78: 449–454
Desilets H, Desjardins Y & Belanger RR (1993) Clonal propagation of Pelargonium X hortorum through tissue culture-Effects of salt dilution and growth regulator concentration. Can. J. Plant Sci. 73: 871–878
Dunbar K & Stephens C (1989) Shoot regeneration of hybrid seed geranium (Pelargonium x hortorum) and regal geranium (Pelargonium x domesticum) from primary callus cultures. Plant Cell Tiss. Org. Cult. 19: 13–21
Evensen KB, Clark DG & Singh A (1993) Rapid ethylene-induced gene expression during petal abscission. In: Pech JC, Latché A & Balagué C (eds) Cellular and Molecular Aspects of the Plant Hormone Ethylene (pp 278–283). Kluwer Academic Publishers, Dordrecht
Gamborg O, Miller R & Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cultures. Expt. Cell Res. 50: 151–158
Grazzini R, Hesk D, Yerger E, Cox-Foster D, Medford J, Craig R & Mumma RO (1995) Species distribution of biochemical and morphological characters associated with small pest resistance in Pelargonium x hortorum. J. Amer. Soc. Hort. Sci. 120: 336–342
Kouider M, Korban S, Skirvin R & Chu M (1984) Influence of embryonic dominance and polarity on adventitious shoot formation from apple cotyledons in vitro. J. Amer. Soc. Hort. Sci. 109: 381–385
Mante S, Scorza R & Cordts J (1989) A simple, rapid protocol for adventitious shoot development from mature cotyledons of Glycine max cultivar Bragg. In Vitro Cell. Dev. Biol. 25: 385–388
Mastalerz JW & Holcomb EJ (1982) Geraniums III. Third Edition. Pennsylvania Flower Growers, University Park, PA
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497
Pillai S & Hildebrandt A (1968) Geranium plants differentiated in vitro from shoot tip and callus cultures. Plant Dis. Rep. 52: 600–601
Power C (1987) Organogenesis from Helianthus annuus inbreds and hybrids from the cotyledons of zygotic embryos. Amer. J. Bot. 74: 497–503
Qureshi JA & Saxena PK (1992) Adventitious shoot induction and somatic embryogenesis with intact seedlings of several hybrid seed geranium (Pelargonium x hortorum Bailey) varieties. Plant Cell Rep. 11: 443–448
Visser C, Qureshi J, Gill R & Saxena P (1992) Morphoregulatory role of thidiazuron. Plant Physiol. 99: 1704–1707
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Chang, C., Moll, B.A., Evenson, K.B. et al. In vitro plantlet regeneration from cotyledon, hypocotyl and root explants of hybrid seed geranium. Plant Cell Tiss Organ Cult 45, 61–66 (1996). https://doi.org/10.1007/BF00043429
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DOI: https://doi.org/10.1007/BF00043429