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Clonal propagation in vitro of red beet (Beta vulgaris ssp.) by multiple adventitious shoot formation

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Abstract

An efficient procedure is described for the rapid in vitro clonal propagation of red beets (Beta vulgaris ssp.) from cultured shoot tips. Propagation was through a tillering response of the rosette plantlets or through multiple adventitious bud formation on the petioles. Histological analysis of petiole sections indicated that buds arise from single cells of the upper epidermis.

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References

  1. Atanasov A, Kikindonov T (1972) Organogenesis of sugarbeet cultures in vitro compared with other species. Genet Sel 5: 399–407

    Google Scholar 

  2. Conger BV ed (1981) Principles and practices of cloning agricultural plants via in vitro techniques. Boca Raton, USA: CRC Press

    Google Scholar 

  3. Coumans-Gilles MF, Kevers C, Coumans M, Ceulemans E, Gaspar T (1981) Vegetative multiplication of sugarbeet through in vitro culture of inflorescence pieces. Plant Cell Tiss Org Cult 1: 93–101

    Google Scholar 

  4. Doll S, Rodier F, Willenbrink J (1979) Accumulation of sucrose in vacuoles isolated from red beet tissue. Planta 144:407–411

    Google Scholar 

  5. Gerlach D (1977) Botanische Mikrotechnik. Stuttgart, FRG: G. Thieme Verlag

    Google Scholar 

  6. de Greef W, Jacobs M (1979) In vitro culture of the sugarbeet: description of a cell line with high regeneration capacity. Plant Sci Lett 17:55–61

    Google Scholar 

  7. Haccius B, Lakshmanan KK (1969) Adventiv-Embryonen-Embryoide-Adventiv-knospen. Ein Beitrag zur Klärung der Begriffe. Oesterr Bot Z 116:145–158

    Google Scholar 

  8. Harms CT, Potrykus I, Widholm JM (1981) Complementation and dominant expression of amino acid analogue resistance markers in somatic hybrid clones from Daucus carota L. after protoplast fusion. Z Pflanzenphysiol 101:377–390

    Google Scholar 

  9. Hooker MP, Nabors MW (1977) Callus initiation, growth, and organogenesis in sugarbeet (Beta vulgaris L.). Z Pflanzenphysiol 84:237–246

    Google Scholar 

  10. Hussey G, Hepher A (1978) Clonal propagation of sugarbeet plants and the formation of polyploids by tissue culture. Ann Bot 42:4–7–479

    Google Scholar 

  11. Larkin PJ, Scowcroft WR (1981) Somaclonal variation-a novel source of variability from cell cultures for plant improvement. Theor Appl Genet 60:197–214

    Google Scholar 

  12. Leigh RA, Branton D (1976) Isolation of vacuoles from root storage tissue of Beta vulgaris L. Plant Physiol 58:656–662

    Google Scholar 

  13. Margana J (1970) Néoformation de bourgeons in vitro chez la betterave sucrière, Beta vulgaris L. C R Acad Sci [Paris] Ser D 270:698–701

    Google Scholar 

  14. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Google Scholar 

  15. van Steveninck RFM (1978) Control of ion transport in plant storage tissue slices. In: Kahl G, ed, Biochemistry of wounded plant tissues. Berlin: W de Gruyter Verlag, pp 503–542

    Google Scholar 

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Harms, C.T., Baktir, I. & Oertli, J.J. Clonal propagation in vitro of red beet (Beta vulgaris ssp.) by multiple adventitious shoot formation. Plant Cell Tiss Organ Cult 2, 93–102 (1983). https://doi.org/10.1007/BF00043354

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  • DOI: https://doi.org/10.1007/BF00043354

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