Abstract
Protoplasts were isolated from aseptic shoot cultures of commercial cultivars ofBrassica napus, B. oleracea andB. campestris, and from the six ‘rapid-cycling brassica species’. Of the rapid-cycling species, onlyB. napus responded well to the culture conditions used; 2% of protoplasts formed calli and up to 5% of calli regenerated shoots. Regeneration was also achieved from commercial cultivars ofB. napus andB. oleracea. For these two species the plating density, time of dilution with fresh medium and the composition of the shoot-inducing medium were all found to have an important influence on the efficiency of plant regeneration. Both responded better to maltose than to sucrose-based media. Under the optimum conditionsB. napus showed a plating efficiency of 7.8% and shooting efficiency of 17%; forB. oleracea the figures were 2% and 56%, respectively.
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Abbreviations
- BAP:
-
6-benzylaminopurine
- NAA:
-
α-naphthaleneacetic acid
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
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Loudon, P.T., Nelson, R.S. & Ingram, D.S. Studies of protoplast culture and plant regeneration from commercial and rapid-cyclingBrassica species. Plant Cell Tiss Organ Cult 19, 213–224 (1989). https://doi.org/10.1007/BF00043348
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DOI: https://doi.org/10.1007/BF00043348