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Carry-over of thigmomorphogenetic characteristics in calli derived fromBryonia dioica internodes

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Abstract

Calli have been initiated in vitro from young internodes (control and rubbed) ofBryonia dioica, where previously it had been shown, using intact plants, that rubbing induced limited growth through enhanced lignification. Calli derived from rubbed internodes were somewhat more compact and showed biochemical changes, i.e. enhanced activity of total peroxidase and isoperoxidases, enhanced production of 1-aminocyclopropane-1-carboxylic acid (ACC) and ethylene, enhanced tissue capacity to convert ACC into ethylene, enhanced activity of phenylalanine ammonia-lyase (PAL) and higher content of lignin, which characterized rubbed internodes. Differences in ethylene metabolism between the two types of calli tended to fade from the third week onwards of initial culture, whereas lignin content, peroxidase activity and peroxidase isoenzyme pattern appeared to be more persistant rubbing-induced markers for several subcultures. The results point to the persistance of environmentally induced changes in gene expression.

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Authors and Affiliations

  1. Laboratoire de Phytomorphogenèse, Université Blaise Pascal, 4 Rue Ledru, F-63038, Clermont-Ferrand, France

    Pascale Bourgeade, Nicole Boyer & Geert de Jaegher

  2. Hormonologie fondamentale et appliquée, Institut de Botanique B22, Université de Liège-Sart Tilman, B-4000, Liège, Belgium

    Thomas Gaspar

Authors
  1. Pascale Bourgeade
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  2. Nicole Boyer
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  3. Geert de Jaegher
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  4. Thomas Gaspar
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Bourgeade, P., Boyer, N., de Jaegher, G. et al. Carry-over of thigmomorphogenetic characteristics in calli derived fromBryonia dioica internodes . Plant Cell Tiss Organ Cult 19, 199–211 (1989). https://doi.org/10.1007/BF00043347

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  • DOI: https://doi.org/10.1007/BF00043347

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