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Preparation of a tRNA-dependent wheat germ protein-synthesizing system

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Abstract

A method is described for the preparation of a tRNA-dependent wheat germ protein-synthesizing system. This system can be supplied with exogenous tRNAs of eukaryotic or prokaryotic origin. In order to obtain maximal aminoacylation of the added tRNAs, the translation assays can be supplemented with homologous aminoacyl-tRNA synthetase preparations. Such a tRNA-dependent wheat germ protein-synthesizing system, which is easy to prepare, can be used not only to translate plant cytoplasmic mRNAs in the presence of added cytoplasmic tRNAs, but also to determine the translation activity of exogenous tRNAs from various sources in the presence of either natural or in vitro synthesized mRNAs.

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Author notes

  1. D. T. N. Pillay

    Present address: Department of Biology, University of Windsor, N9B 3P4, Ontario, Canada

  2. P. Guillemaut

    Present address: Division de Biologie Appliquée, Institut Universitaire de Technologie Louis Pasteur, 3 rue de l'Argonne, 67000, Strasbourg, France

Authors and Affiliations

  1. Institut de Biologie Moléculaire des Plantes, Université Louis Pasteur, 12 rue du Général Zimmer, 67000, Strasbourg, France

    H. Pfitzinger, J. H. Weil, D. T. N. Pillay & P. Guillemaut

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  1. H. Pfitzinger
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  2. J. H. Weil
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  3. D. T. N. Pillay
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  4. P. Guillemaut
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Pfitzinger, H., Weil, J.H., Pillay, D.T.N. et al. Preparation of a tRNA-dependent wheat germ protein-synthesizing system. Plant Mol Biol 12, 301–306 (1989). https://doi.org/10.1007/BF00043207

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  • DOI: https://doi.org/10.1007/BF00043207

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