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Changes in protein and protease activity during the growth and senescence of Paul's Scarlet rose

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Abstract

A study was performed to determine the relationship between the protein content and protease activity in suspension cultures of rose (Rosa cv Paul's Scarlet) grown over a 30 day period. Protein levels and protease activity were calculated on a per culture and per cell basis. Older nongrowing 14 day-old cultures possessed the largest total protease activity, but the highest concentration of protease activity per cell was in young 4 day-old rapidly dividing cells.

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References

  1. Boller T, Kende H (1979) Hydrolytic enzymes in the central vacuole of plant cells. Plant Physiol 63: 1123–1132

    Google Scholar 

  2. Carlberg I, Soderhall K, Glimelius K, Eriksson T (1984) Protease activities in non-embryogenic and embryogenic carrot cell strains during callus growth and embryo formation. Physiol Plant. 62: 458–464

    Google Scholar 

  3. Medora RS, Mell GP, Bilderback DE (1984) Effect of various media on growth and protease production in Carica papaya L. callus cultures. Z. Pflanzenphysiol 114: 179–185

    Google Scholar 

  4. Mell GP, Medora RS, Bilderback DE (1979) Substrate specificity of enzymes from papaya callus cultures. Z. Pflanzenphysiol 91: 279–282

    Google Scholar 

  5. Mohanty B, Fletcher JS (1978) Influence of ammonium on the growth and development of suspension cultures of Paul's Scarlet rose. Physiol Plant 42: 221–225

    Google Scholar 

  6. Nesius KK, Uchytil LE, Fletcher JS (1972) Minimal organic medium for suspension cultures of Paul's Scarlet rose. Planta 106: 173–176

    Google Scholar 

  7. Ragster L, Chrispeels MJ (1979) Azocoll-digesting proteinases in soybean leaves. Plant Physiol 64: 857–862

    Google Scholar 

  8. Reider ML, Smith BA, Fletcher JS (1982) Protein content and subculturing properties of senescing plant suspension cultures. In Vitro 82: 1004–1008

    Google Scholar 

  9. Samac D, Storey R (1981) Proteolytic and trypsin inhibitor activity in germinating jojoba seeds (Simmondsia chinensis). Plant Physiol 68: 1339–1344

    Google Scholar 

  10. Smith BA, Reider ML, Fletcher JS (1982) Relationship between vital staining and subculture growth during the senescence of plant tissue cultures. Plant Physiol 70: 1228–1230

    Google Scholar 

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Reider, M.L., Fletcher, J.S. Changes in protein and protease activity during the growth and senescence of Paul's Scarlet rose. Plant Cell Tiss Organ Cult 7, 115–119 (1986). https://doi.org/10.1007/BF00043035

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  • DOI: https://doi.org/10.1007/BF00043035

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