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In vitro propagation of Halesia carolina L. and the influence of explantation timing on initial shoot proliferation

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Abstract

Halesia carolina L., a small, ornamentally valuable tree, is difficult to propagate due to the complexity of seed propagation and the unavailability of propagules for conventional vegetative propagation. A micropropagation system was developed to facilitate easy propagation of this species. Actively growing shoot tips achieved optimum shoot proliferation from axillary buds when placed on Woody Plant Medium supplemented with 1.0 to 2.5 mg/l benzyladenine. The addition of 0.1 mg/l naphthaleneacetic acid had little effect on culture performance. Murashige and Skoog medium was incapable of supporting vigorous shoot proliferation. Non-sterile rooting conditions provided better rooting and subsequent plantlet growth, when compared to an in vitro rooting method. The seasonal fluctuations in the stock plant dramatically affected the shoot proliferating potential of the explants in vitro. Rapidly elongating shoots formed shoot proliferating cultures more slowly than explants taken either before or after the rapid elongation phase.

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Authors and Affiliations

  1. Department of Horticulture, The Ohio Agricultural Research and Development Center, The Ohio State University, 43210, Columbus, OH, USA

    Mark H. Brand & R. Daniel Lineberger

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  1. Mark H. Brand
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  2. R. Daniel Lineberger
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Brand, M.H., Lineberger, R.D. In vitro propagation of Halesia carolina L. and the influence of explantation timing on initial shoot proliferation. Plant Cell Tiss Organ Cult 7, 103–113 (1986). https://doi.org/10.1007/BF00043034

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  • DOI: https://doi.org/10.1007/BF00043034

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