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Direct plant regeneration from leaf explants of Drosera rotundifolia cultured in vitro

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Shoot regeneration was obtained from isolated leaves of Drosera rotundifolia L. cultured on MS media with various concentrations of 6-benzyladenine (BA) and α-naphthaleneacetic acid (NAA). The best direct shoot organogenesis was obtained on growth regulator-free medium or medium supplemented with 10-8 M NAA. Liquid culture medium significantly increased regeneration capacity of leaf tissue. Histological and scanning electron microscopy investigations verify direct plant regeneration without intermediate callus formation. Leaf epidermal cells showed the highest regeneration potential leading to the regeneration of buds. Young shoots with three to seven leaflets rooted spontaneously on the growth regulator-free medium within 38 days of culture and isolated mature plants produced fertile seeds.

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40% formalin (5%) +90% acetic acid (5%) +70% ethanol (90%)


Murashige and Skoog's (1962) medium


α-naphthaleneacetic acid






scanning electron microscopy


transmission electron microscopy


photosynthetic photon flux


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Bobák, M., Blehová, A., Krištín, J. et al. Direct plant regeneration from leaf explants of Drosera rotundifolia cultured in vitro . Plant Cell Tiss Organ Cult 43, 43–49 (1995).

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