Skip to main content
Log in

Direct plant regeneration from leaf explants of Drosera rotundifolia cultured in vitro

  • Original Research Papers
  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

Shoot regeneration was obtained from isolated leaves of Drosera rotundifolia L. cultured on MS media with various concentrations of 6-benzyladenine (BA) and α-naphthaleneacetic acid (NAA). The best direct shoot organogenesis was obtained on growth regulator-free medium or medium supplemented with 10-8 M NAA. Liquid culture medium significantly increased regeneration capacity of leaf tissue. Histological and scanning electron microscopy investigations verify direct plant regeneration without intermediate callus formation. Leaf epidermal cells showed the highest regeneration potential leading to the regeneration of buds. Young shoots with three to seven leaflets rooted spontaneously on the growth regulator-free medium within 38 days of culture and isolated mature plants produced fertile seeds.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Abbreviations

BA:

6-benzyladenine

FAA:

40% formalin (5%) +90% acetic acid (5%) +70% ethanol (90%)

ME:

Murashige and Skoog's (1962) medium

NAA:

α-naphthaleneacetic acid

plumbagin:

5-hydroxy-2-methyl-1,4-naphthoquinone

7-methyljuglone:

7-methyl-5-hydroxy-1,4-naphthoquinone

SEM:

scanning electron microscopy

TEM:

transmission electron microscopy

PPF:

photosynthetic photon flux

References

  • Anthony JL (1992) In vitro propagation of Drosera sp. HortScience 27: 850

    Google Scholar 

  • Blehová A, Erdelský K, Bobák M (1992) Cultivation of organ and callus culture of Drosera spathulata Labill. in vitro conditions. Acta F.R.N. Univ. Comen. Physiol. Plant. 27: 93–102

    Google Scholar 

  • Bobák M, Blehová A & Erdelský K (1989) Histological and cytological studies of early phases of organogenesis on excised leaves of Drosera spathulata Labill. in vitro. Biologia 44: 785–792

    Google Scholar 

  • Bobák M, Blehová A, Šamaj J, Ovečka M & Krištín J (1993) Studies of organogenesis from the callus culture of sundew (Drosera spathulata Labill.). J. Plant Physiol. 142: 251–253

    Google Scholar 

  • Bonnet M, Coumans M, Hofinger M, Ramaut JL & Gaspar T (1984) Vegetative multiplication in vitro of the sundew Drosera rotundifolia. Arch. Int. Physiol. Biochem. 92: 16–17

    Google Scholar 

  • Conger BV, Hanning GE, Gray DJ & McDaniel JK (1983) Direct embryogenesis from mesophyll cells of orchardgrass. Science 221: 850–851

    Google Scholar 

  • Crouch IJ, Finnie JF & Van Staden J (1990) Studies on the isolation of plumbagin from in vitro and in vivo grown Drosera species. Plant Cell Tiss. Org. Cult. 21: 78–82

    Google Scholar 

  • Dubois T, Guediva M, Dubois J, Vasseur J (1991) Direct somatic embryogenesis in leaves of Cichorium. A. histological and SEM study of early stages. Protoplasma 162: 120–127.

    Google Scholar 

  • Fetterer RH & Fleming MW (1991) Effects of plumbagin on development of the parasitic nematodes Heamonchus contortus and Ascaris suum. Comp. Biochem. Physiol. 100: 539–542

    Google Scholar 

  • Fujii N, Yamashita Y, Arima Y, Nagashima M & Nakano H (1992) Induction of Topoisomerase II — mediated DNA cleavage by the plant naphtoquinones plumbagin and shikonin. Antimicrob. Agents Chemotherapy 36: 2589–2594

    Google Scholar 

  • Konar RN & Natarja K (1965) Experimental studies in Ranunculus scleratus L. Development of embryos from the stem epidermis. Phytomorphology 15: 132–137.

    Google Scholar 

  • McWilliam AA, Smith SM & Street HE (1974) The origin and development of embryoids in suspension cultures of carrot (Daucus carota). Ann. Bot. 38: 243–250.

    Google Scholar 

  • Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497

    Google Scholar 

  • Pihakaski-Maunbach K, Brauner Nygaard K, Tensen KH & Rasmussen O (1993) Cellular changes in early development of regenerating thin cell layer-explants of rapeseed analyzed by light and electron microscopy. Physiol. Plant. 87: 167–176

    Google Scholar 

  • Ruoslahti E & Pierschbacher MD (1987) New perspectives in cell adhesion: RGD and integrins. Science 238: 491–497

    Google Scholar 

  • Šamaj J, Bobák M, Blehová A, Krštín J, Auxtová-Šamajová O (1995) Developmental SEM observation of an extracellular matrix in embryogenic calli of Drosera rotundifolia and Zea mays. Protoplasma 186: 45–49

    Google Scholar 

  • Schölly T & Kapetanidis I (1989) Droseron-5-glucosid — ein neues Heterosid aus den oberirdischen Teilen von Drosera rotundifolia L. (Droseraceae). Pharm. Acta Helv. 64: 66–67

    Google Scholar 

  • Simola LK (1978) The effect of several amino acids and some inorganic nitrogen sources on the growth of Drosera rotundifolia in long and short-day conditions. Z. Pflanzenphysiol. 90: 61–68

    Google Scholar 

  • Stolarz A, Macewicz J & Lorz H (1991) Direct somatic embryogenesis and plant regeneration from leaf explants of Nicotiana tabacum L. J. Plant Physiol. 137: 347–357.

    Google Scholar 

  • Young R, Kaul V, Williams E G (1987) Clonal propagation in vitro from immature embryos and flower buds of Lycopersion peruviatum and L. esculentum. Plant Sci. 52: 237–242

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bobák, M., Blehová, A., Krištín, J. et al. Direct plant regeneration from leaf explants of Drosera rotundifolia cultured in vitro . Plant Cell Tiss Organ Cult 43, 43–49 (1995). https://doi.org/10.1007/BF00042670

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00042670

Key words

Navigation