Abstract
Zygotic embryos, stratified and collected at 8, 16 and 24 weeks and cotyledonary and epicotyl explants from 30 and 45 day old Cycas revoluta Thumb. seedlings were cultured in vitro to determine their morphogenic potential. Depending upon the duration of stratification and growth regulator type, zygotic embryos exhibited a different morphogenic response. The youngest embryos rarely survived, while larger ones formed a callus, depending on the growth regulator in the medium, and the oldest ones produced putative embryogenic masses. Cytokinin levels double the auxin concentrations resulted in shoot regeneration in almost mature embryos. In addition, in vitro culture of younger cotyledonary explants gave shoot regeneration, while older ones formed adventitious roots. The effectiveness of BA in inducing shoot regeneration from seedling explants was confirmed. NAA in combination with cytokinin in the induction medium inhibited bud induction. These results show that the morphogenic potential of tissues is linked to their developmental stage.
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Abbreviations
- BA:
-
benzyladenine
- 2,4-d :
-
2,4-dichlorophenoxyacetic acid
- 2iP:
-
2-isopentenyladenine
- NAA:
-
α naphthaleneacetic acid
- SH:
-
Schenk & Hildebrandt medium (1972)
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Rinaldi, L.M.R., Leva, A.R. In vitro organogenesis from diploid tissues of Cycas revoluta Thunb.. Plant Cell Tiss Organ Cult 43, 37–41 (1995). https://doi.org/10.1007/BF00042669
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DOI: https://doi.org/10.1007/BF00042669