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Plant Molecular Biology

, Volume 23, Issue 6, pp 1279–1284 | Cite as

Molecular characterization of multiple cDNA clones for ADP-glucose pyrophosphorylase from Arabidopsis thaliana

  • Per Villand
  • Odd-Arne Olsen
  • Leszek A. Kleczkowski
Short Communication

Abstract

PCR amplification of cDNA prepared from poly(A)+ RNA from aerial parts of Arabidopsis thaliana, using degenerate nucleotide primers based on conserved regions between the large and small subunits of ADP-glucose pyrophosphorylase (AGP), yielded four different cDNAs of ca. 550 nucleotides each. Based on derived amino acid sequences, the identities between the clones varied from 49 to 69%. Sequence comparison to previously published cDNAs for AGP from various species and tissues has revealed that three of the amplified cDNAs (ApL1, ApL2 and ApL3) correspond to the large subunit of AGP, and one cDNA (ApS) encodes the small subunit of AGP. Both ApL1 and ApS were subsequently found to be present in a cDNA library made from Arabidopsis leaves. All four PCR products are encoded by single genes, as found by genomic Southern analysis.

Key words

ADP-glucose pyrophosphorylase Arabidopsis thaliana starch biosynthesis 

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Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • Per Villand
    • 1
  • Odd-Arne Olsen
    • 1
  • Leszek A. Kleczkowski
    • 1
  1. 1.Plant Molecular Biology LaboratoryAgricultural Research Council of NorwayÅsNorway

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