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Plant Molecular Biology

, Volume 23, Issue 6, pp 1255–1264 | Cite as

Characterization of Synechocystis sp. PCC 6803 in iron-supplied and iron-deficient media

  • William R. Odom
  • Reneé Hodges
  • Parag R. Chitnis
  • James A. Guikema
Research Article

Abstract

The photosynthetic apparatus is rich in iron-containing cofactors and iron deficiency causes severe impairment of photosynthesis in plants, algae, and cyanobacteria. Synechocystis sp. PCC 6803 serves as a model system to investigate the complex assembly and integration of the multi-subunit protein complexes of oxygenic photosynthetic electron transport; particularly when coupled to developmental cues due to nutrient limitation or requirements. We study Fe3+-deficient and Fe3+-supplemented cultures of Synechocystis sp. PCC 6803. The autotrophic growth rate of Fe3+-deficient cultures is slower than Fe3+-supplemented cultures. Whole cell spectral analysis reveals differences in both the quantity and the peak absorbance of chlorophyll. Fe3+ deficiency decreases rates of photosynthetic electron transport and the mRNA and corresponding protein levels as observed using specific probes. mRNA levels of psaB increased 20-fold during recovery from Fe3+ deficiency, as compared to the control. psaD transcript levels increased to 160% during recovery as compared to the control. PsaA/B heterodimer formation and turnover is dependent on Fe3+ and the complete assembly on the reducing side of photosystem I (PS I) is PsaD-dependent. Recovery from Fe3+ deficiency suggests that regulation occurs at both the mRNA and protein level.

Key words

Fe3+-deficient integration iron-sulfur center mRNA levels photosystem I (PS I) Synechocystis 

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Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • William R. Odom
    • 1
  • Reneé Hodges
    • 1
  • Parag R. Chitnis
    • 1
  • James A. Guikema
    • 1
  1. 1.Division of BiologyKansas State UniversityManhattanUSA

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