Advertisement

Plant Molecular Biology

, Volume 23, Issue 6, pp 1211–1221 | Cite as

Two related, low-temperature-induced genes from Brassica napus are homologous to the human tumour bbc1 (breast basic conserved) gene

  • Julio Sáez-Vásquez
  • Monique Raynal
  • Luis Meza-Basso
  • Michel Delseny
Research Article

Abstract

In order to identify genes involved in cold acclimation, we have constructed a cDNA library from Brassica napus (cv. Samouraï) cold-acclimated etiolated seedlings. By differential screening, a cDNA clone named pBnC24 (Brassica napus Cold), corresponding to a new cold-inducible plant gene, was isolated. Northern blot hybridizations using total RNA from acclimated and unacclimated seedlings confirmed that BnC24 represents a cold-regulated gene. In contrast with a number of cold-inducible plant genes, BnC24 does not seem to be responsive to abscisic acid (ABA). In addition, further screening of the ‘cold-acclimated’ cDNA library using pBnC24 cDNA as a probe, allowed the isolation of a second type of homologous cDNA. Sequence analysis showed that the two BnC24 genes encode basic 24 kDa proteins, which are highly hydrophilic and rich in alanine, lysine and arginine. The nucleotide and deduced amino acid sequences of these clones do not show any homology with other previously described cold-induced plants genes. However they have strong homology with a recently discovered human tumour gene, bbc1 (breast basic conserved), which seems to be highly conserved in eukaryotes.

Key words

Brassica napus cold acclimation gene isolation human tumour 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Adams, SM, Helps, NR, Sharp, MGS, Brammar, WJ, Walker, RA, Varley, JM: Isolation and characterization of a novel gene with differential expression in benign and malignant human breast tumours. Hum Mol Genet: 1: 91–96 (1992).Google Scholar
  2. 2.
    Baker, J, Stoele, C, DureIII, L: Sequence and characterization of 6 LEA proteins and their genes from cotton. Plant Mol Biol 11: 277–291 (1988).Google Scholar
  3. 3.
    Cativelli, L, Bartels, D: Molecular cloning and characterization of cold-regulated genes in barley. Plant Physiol 93: 1504–1510 (1990).Google Scholar
  4. 4.
    Chen, Y, Seeburg, PY: Supercoil sequencing: A fast and simple method for sequencing plasmid DNA. DNA 4: 165–190 (1985).Google Scholar
  5. 5.
    Delseny, M, Gaubier, P, Hull, G, Sáez-Vásquez, J, Gallois, P, Raynal, M, Cooke, R, Grellet, F: Nuclear genes expressed during seed desiccation: relationship with responses to stress. In: Basra, AS (ed) Stress Induced Gene Expression. Harwood Academic Publishers, Reading, UK, in press (1993).Google Scholar
  6. 6.
    Dunn, MA, Hughes, MA, Zhang, L, Pearce, RS, Quigley, AS, Jack, PL: Nucleotide sequence and molecular analysis of the low temperature induced cereal gene, BLT4. Mol Gen Genet 229: 389–394 (1991).Google Scholar
  7. 7.
    Gilmour, SJ, Artus, NN, Thomashow, MF: cDNA sequence analysis and expression of two cold-regulated genes of Arabidopsis thaliana. Plant Mol Biol 18: 13–21 (1992).Google Scholar
  8. 8.
    Grellet, F, Delcasso-Tremousaygue, D, Delseny, M: Isolation and characterization of an unusual repeated sequence from the ribosomal intergenic spacer of the crucifer Sisymbrium irio. Plant Mol Biol 12: 695–706 (1989).Google Scholar
  9. 9.
    Guo, W, Ward, RW, Thomashow, MF: Characterization of a cold regulated wheat gene related to Arabidopsis cor 47. Plant Physiol 100: 915–922 (1992).Google Scholar
  10. 10.
    Griffith, M, Ala, P, Yang, DSC, Hon, WC, Moffat, BA: Antifreeze protein produced endogenously in winter rye leaves. Plant Physiol 100: 593–596 (1992).Google Scholar
  11. 11.
    Guy, CL: Cold acclimation and freezing stress tolerance: role of protein metabolism. Annu Rev Plant Physiol Plant Mol Biol 41: 187–223 (1990).Google Scholar
  12. 12.
    Houde, M, Danyluk, J, Laliberté, JF, Rassat, E, Dhindsa, RS, Sarhan, F: Cloning, characterization and expression of a cDNA encoding a 50-kilodalton protein specifically induced by cold acclimation in wheat. Plant Physiol 99: 1381–1387 (1992).Google Scholar
  13. 13.
    Hugues, MA, Dunn, MA, Pearce, RS, White, AJ, Zhang, L: An abscisic-acid-responsive, low temperature barley gene has homology with a maize phospholipid transfer protein. Plant Cell Environ 15: 861–865 (1992).Google Scholar
  14. 14.
    Jarillo, JA, Leyva, A, Salinas, J, Martinez-Zapater, JM: Low temperature induces the accumulation of alcohol dehydrogenase mRNA in Arabidopsis thaliana, a chilling-tolerant plant. Plant Physiol 101: 833–837 (1993).Google Scholar
  15. 15.
    Kurkela, S, Borg-Franck, M: Structure and expression of kin 2, one of two cold and ABA-induced genes of Arabidopsis thaliana. Plant Mol Biol 19: 689–692 (1992).Google Scholar
  16. 16.
    Lang, V, Palva, ET: The expression of a rab-related gene, rab 18, is induced by abscisic acid during the cold acclimation process of Arabidopsis thaliana (L.) Heynh. Plant Mol Biol 20: 951–962 (1992).Google Scholar
  17. 17.
    Lee, SP, Chen, THH: Molecular cloning of abscisic acid-responsive mRNAs expressed during the induction of freezing tolerance in bromegrass (Bromus inermis Leyss) suspension culture. Plant Physiol 101: 1089–1096 (1993).Google Scholar
  18. 18.
    Lin, C, Thomashow, MF: DNA sequence analysis of a complementary DNA for cold-regulated Arabidopsis gene cor15 and characterization of the COR 15 polypeptide. Plant Physiol 99: 519–525 (1992).Google Scholar
  19. 19.
    Luo, M, Liu, JH, Mohapatra, S, Hill, RD, Mohapatra, SS: Characterization of gene family encoding abscisic acid-and environmental stress-inducible proteins of alfalfa. J Biol Chem 267: 15 367–15 374 (1992).Google Scholar
  20. 20.
    Maniatis, T, Fritsch, EF, Sambrook, J: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (1982).Google Scholar
  21. 21.
    Meza-Basso, L, Alberdi, M, Raynal, M, Ferrero-Cadinanos, M-L, Delseny, M: Changes in protein synthesis in rapeseed (Brassica napus) seedlings during a low temperature treatment. Plant Physiol 82: 733–738 (1986).Google Scholar
  22. 22.
    Nordin, K, Heino, P, Palva, T: Separate signal pathways regulate the expression of a low-temperature-induced gene in Arabidopsis thaliana (L.) Heynh. Plant Mol Biol 16: 1061–1071 (1991).Google Scholar
  23. 23.
    Nordin, K, Vahala, T, Palva, T: Differential expression of two related, low-temperature-induced genes in Arabidopsis thaliana (L.) Heynh. Plant Mol Biol 21: 641–653 (1993).Google Scholar
  24. 24.
    Parra, C, Sáez, J, Pérez, H, Alberdi, M, Delseny, M, Hubert, E, Meza-Basso, L: Cold resistance in rapeseed (Brassica napus) seedlings. Searching biochemical markers of cold-tolerance. Arch Biol Med Exp 23: 187–194 (1990).Google Scholar
  25. 25.
    Pearson, WR: Rapid and sensitive sequence comparison with FASTAP and FASTA. Meth Enzymol 183: 63–93 (1990).Google Scholar
  26. 26.
    Raikhel, N: Nuclear targeting in plants. Plant Physiol 100: 1627–1632 (1992).Google Scholar
  27. 27.
    Rosas, A, Alberdi, M, Delseny, M, Meza-Basso, L: A cryoprotective polypeptide isolated from Nothogagus dombeyi seedlings. Phytochemistry 25: 2497–2500 (1986).Google Scholar
  28. 28.
    Steponkus, PL: Role of the plasma membrane in freezing injury and cold acclimation. Annu Rev Plant Physiol 35: 543–584 (1984).Google Scholar
  29. 29.
    Sutton, F, Ding, X, Kenefick, DG: Group 3 LEA gene HVA1 regulation by cold acclimation and deacclimation in two barley cultivars with varying freeze resistance. Plant Physiol 99: 338–340 (1992).Google Scholar
  30. 30.
    Thomashow, MF: Molecular genetics of cold acclimation in higher plants. Adv Genet 28: 99–131 (1990).Google Scholar
  31. 31.
    Tognetti, J, Salerno, GL, Crespi, MD, Pontis, H: Surcrose and fructan metabolism of different wheat cultivars at chilling temperatures. Physiol Plant 78: 554–559 (1990).Google Scholar
  32. 32.
    Weretilnyk, E, Orr, W, White, TC, Iu, B, Singh, J: Characterization of three related low-temperature-regulated cDNAs from winter Brassica napus. Plant Physiol 101: 171–177 (1993).Google Scholar
  33. 33.
    Zhu, B, Chen, THH, Li, PH: Expression of an ABA-responsive osmotin-like gene during the induction of freezing tolerance in Solanum commersonii. Plant Mol Biol 21: 729–735 (1993).Google Scholar

Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • Julio Sáez-Vásquez
    • 1
    • 2
  • Monique Raynal
    • 1
  • Luis Meza-Basso
    • 2
  • Michel Delseny
    • 1
  1. 1.Laboratoire de Physiologie et Biologie Moléculaire Végétales, URA 565 du CNRSUniversité de PerpignanPerpignan-CedexFrance
  2. 2.Departamento de Ciencias Biologicas, Programa de Biología vegetalUniversidad de TalcaTalcaChile

Personalised recommendations