Plant Molecular Biology

, Volume 23, Issue 6, pp 1211–1221 | Cite as

Two related, low-temperature-induced genes from Brassica napus are homologous to the human tumour bbc1 (breast basic conserved) gene

  • Julio Sáez-Vásquez
  • Monique Raynal
  • Luis Meza-Basso
  • Michel Delseny
Research Article


In order to identify genes involved in cold acclimation, we have constructed a cDNA library from Brassica napus (cv. Samouraï) cold-acclimated etiolated seedlings. By differential screening, a cDNA clone named pBnC24 (Brassica napus Cold), corresponding to a new cold-inducible plant gene, was isolated. Northern blot hybridizations using total RNA from acclimated and unacclimated seedlings confirmed that BnC24 represents a cold-regulated gene. In contrast with a number of cold-inducible plant genes, BnC24 does not seem to be responsive to abscisic acid (ABA). In addition, further screening of the ‘cold-acclimated’ cDNA library using pBnC24 cDNA as a probe, allowed the isolation of a second type of homologous cDNA. Sequence analysis showed that the two BnC24 genes encode basic 24 kDa proteins, which are highly hydrophilic and rich in alanine, lysine and arginine. The nucleotide and deduced amino acid sequences of these clones do not show any homology with other previously described cold-induced plants genes. However they have strong homology with a recently discovered human tumour gene, bbc1 (breast basic conserved), which seems to be highly conserved in eukaryotes.

Key words

Brassica napus cold acclimation gene isolation human tumour 


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Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • Julio Sáez-Vásquez
    • 1
    • 2
  • Monique Raynal
    • 1
  • Luis Meza-Basso
    • 2
  • Michel Delseny
    • 1
  1. 1.Laboratoire de Physiologie et Biologie Moléculaire Végétales, URA 565 du CNRSUniversité de PerpignanPerpignan-CedexFrance
  2. 2.Departamento de Ciencias Biologicas, Programa de Biología vegetalUniversidad de TalcaTalcaChile

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