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Regeneration of plants from Antirrhinum majus L. callus

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Abstract

Somaclone production in Antirrhinum majus plants by regeneration of plants from callus cultures has been achieved using three types of explant tissue. Regeneration from mature stem internode-derived callus was extremely poor. Callus derived from seedling shoot tips could be induced to form new shoots in six of seven cultivars tested. Regeneration was achieved in all seven cultivars when callus was produced from segments of hypocotyl and was most effective using agar-solidified medium containing 0.25 mgl-1 naphthoxyacetic acid + 10% coconut milk. In this case, five of the cultivars produced shoots directly, one produced leaves from the petioles of which new shoots emerged, and one regenerated plants chiefly through the production of embryoids.

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Authors and Affiliations

  1. Department of Plant Biology, University of Birmingham, P.O. Box 363, B15 2TT, Birmingham, UK

    Nicola J. Atkinson, Brian V. Ford-Lloyd & H. John Newbury

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  1. Nicola J. Atkinson
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  2. Brian V. Ford-Lloyd
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  3. H. John Newbury
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Atkinson, N.J., Ford-Lloyd, B.V. & John Newbury, H. Regeneration of plants from Antirrhinum majus L. callus. Plant Cell Tiss Organ Cult 17, 59–70 (1989). https://doi.org/10.1007/BF00042282

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  • DOI: https://doi.org/10.1007/BF00042282

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